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The book is intended to provide a sound basis for enzyme reactor design based on kinetic principles, and give an updated vision of the potentials and limitations of enzyme biocatalysis, especially with respect to recent applications in processes of organic synthesis. The book is structured in the form of a textbook that goes from basic principles of enzyme structure and function to reactor design for homogeneous systems with soluble enzymes, and heterogeneous systems with insolubilized enzymes. It contains an introductory chapter that gives an updated overview of enzyme technology, a chapter on enzyme production focused on large-scale operations, two chapters devoted to homogeneous and heterogeneous enzyme kinetics and a chapter on enzyme reactor design and operation based on enzyme kinetics principles, mass transfer limitations and enzyme inactivation. The book is complemented with case studies of biocatalytic processes of industrial relevance or potential, written by experts in those fields. Applications of proteases, acylases, lipases, aldolases and dehydrogenases in reactions of organic synthesis, and peroxidases in the degradation of recalcitrant organic compounds, are used as selected examples to illustrate the realities and potentials of enzymes as process catalysts.

Table of contents

 

1. Introduction (Andrés Illanes)

2. Enzyme Production (Andrés Illanes)

3. Homogeneous Enzyme Kinetics (Andres Illanes, Claudia Altamirano, Lorena Wilson)

4. Heterogeneous Enzyme Kinetics (Andres Illanes, Roberto Fernández-Lafuente, José Manuel Guisán, Lorena Wilson)

5. Enzyme Reactors (Andrés Illanes, Claudia Altamirano)

6. Study Cases of Enzymatic Processes

6.1. Proteases as catalysts for peptide synthesis (Sonia Barberis, Fanny Guzmán, Josep López-Santín)

6.2. Synthesis of ß-lactam antibiotics with penicillin acylases (Andrés Illanes, Lorena Wilson)

6.3. Chimioselective esterification of wood sterols with lipases (Gregorio Alvaro, Andrés Illanes)

6.4. Oxidoreductases as powerful biocatalysts for green chemistry José Manuel Guisán, Roberto Fernández-Lafuente, Lorena Wilson, César Mateo)

6.5. Use of aldolases for asymmetric synthesis (Josep López-Santín, Gregorio Alvaro, Pere Clapés)

6.6. Application of enzymatic reactors for the degradation of highly and poorly soluble recalcitrant compounds (Juan M. Lema et al.)