1. Viral Genome Packaging Machines: An Overview 1
Carlos Enrique Catalano
2. Bacteriophage Lambda Terminase and the Mechanisms
of Viral DNA Packaging 5
Michael Feiss and Carlos Enrique Catalano
Bacteriophage Lambda Infection and DNA Replication 5
Overview of DNA Packaging 6
Bacteriophage l cos: A Multipartite Assembly Site 8
Components of the Packaging Machinery 11
Procapsid Assembly 20
A Working Model for Lambda DNA Packaging 21
Initiation of Packaging 22
Transition to a Packaging Machine 24
DNA Translocation: Active DNA Packaging 28
Termination of Packaging 30
Virion Completion 33
3. DNA Packaging in Bacteriophage T4 40
Venigalla B. Rao and Lindsay W. Black
Components of the Phage T4 DNA Packaging Machine 42
Gp17: The Large Terminase Protein 44
Activities Associated with gp17 46
Gp20: The Portal Protein 48
Major Events during Phage T4 DNA Packaging 49
Interactions of DNA Packaging with Other DNA Processes 53
DNA Structural Requirements for Packaging 53
Discontinuous Headful Packaging 54
4. T3/T7 DNA Packaging 59
Philip Serwer
Genetics/Genome Sequence 63
Structure of Capsids 68
Biochemistry 71
5. DNA Packaging by Bacteriophage P22 80
Sherwood Casjens and Peter Weigele
Building a Protective Shell for Viral DNA 80
DNA Packaging Strategy 81
Initiation of DNA Packaging 82
Filling the Capsid with DNA 84
Termination of DNA Packaging 85
6. Bacteriophage SPP1 DNA Packaging 89
Anja DrÜge and Paulo Tavares
Assembly of the SPP1 Procapsid, the Proteinaceous
DNA Container 89
Selective Recognition and Cleavage of SPP1 DNA
by the Terminase Complex gp1-gp2 91
Assembly of the DNA Packaging Machine, DNA Translocation,
and Capsid Expansion 95
Termination of DNA Packaging: The Headful Cleavage 96
Stabilization of Packaged DNA and Control of DNA Release.
Assembly and Structure of the SPP1 Connector 97
Processivity of DNA Packaging Events during SPP1 Infection 98
SPP1-Mediated Transduction: Packaging of Host DNA 99
7. The 29 DNA Packaging Motor: Seeking the Mechanism 102
Dwight Anderson and Shelley Grimes
Components of the 29 DNA Packaging Motor 102
The DNA Packaging Assay and Provisional Packaging Events 107
A Model of the Packaging Mechanism 108
The 29 Packaging Motor Is Processive and Powerful 110
Ongoing Analysis of 29 DNA Packaging:
The Path to Enlightenment 113
8. Encapsidation of the Segmented Double-Stranded RNA Genome
of Bacteriophage f6 117
Minna M. Poranen, Markus J. Pirttimaa and Dennis H. Bamford
Components of the f6 RNA Packaging and Replication System 121
Procapsid to Core Transition 123
The f6 in Vitro ssRNA Packaging and Replication System 123
Sequential Packaging 123
Initiation of Minus-Strand Synthesis-A Checkpoint
for Genome Packaging 125
Symmetry Mismatch in the Procapsid of f6 129
A Special Packaging Vertex 129
Comparison to Tailed dsDNA Bacteriophages and Eukaryotic
dsRNA Viruses 130
9. Cleavage and Packaging of Herpes Simplex Virus 1 DNA 135
Joel D. Baines and Sandra K. Weller
Arrangement of the HSV Genome and Packaging Sequences 136
Capsid Maturation 137
Components of the Packaging Machinery 140
The Portal Vertex (UL6) 140
UL25 Protein 141
The Putative Terminase (UL15, UL28, UL33) 142
Intranuclear Transport Proteins (UL17, UL32) 144
Model of DNA Cleavage and Packaging 145
Index 151