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Eine "Werkzeugkiste" für den Biotechniker! Systematisch und praxisorientiert bietet dieses außergewöhnlich umfangreiche, zweibändige Handbuch eine Fülle an technischen, methodischen und anwendungsspezifischen Informationen zum Gesamtgebiet des Protein Engineering.

 

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VOLUME 1

 

Guidelines for the Functional Analysis of Engineered and Mutant Enzymes

Engineering Enantioselectivity in Enzyme-Catalyzed Reactions

Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes

Phi-Value Analysis of Protein Folding Transition States

Protein Folding and Solubility: Pathways and High-Throughput Assays

Protein Dynamics and the Evolution of Novel Protein Function

Gaining Insight into Enzyme Function through Correlation with Protein Motions

Structural Frameworks Suitable for Engineering

Microbes and Enzymes: Recent Trends and New Directions to Expand Protein Space

Inteins in Protein Engineering

From Prospecting to Product - Industrial Metagenomics Is Coming of Age

Computational Protein Design

Assessing and Exploiting the Persistence of Substrate Ambiguity in Modern Protein Catalysts

Designing Programmable Protein Switches

The Cyclization of Peptides and Proteins with Inteins

 

 

VOLUME 2

 

A Method for Rapid Directed Evolution

Evolution of Enantioselective Bacillus subtilis Lipase

Circular Permutation of Proteins

Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): A Versatile Tool for Generating Targeted Libraries

Protein Engineering by Structure-Guided SCHEMA Recombination

Chimeragenesis in Protein Engineering

Protein Generation Using a Reconstituted System

Equipping in vivo Selection Systems with Tunable Stringency

Protein Engineering by Phage Display

Screening Methodologies for Glycosidic Bond Formation

Yeast Surface Display in Protein Engineering and Analysis

In Vitro Compartmentalization (IVC) and Other High-Throughput Screens of Enzyme Libraries

Colorimetric and Fluorescence-Based Screening

Confocal and Conventional Fluorescence-Based High Throughput Screening in Protein Engineering

30 Alteration of Substrate Specificity and Stereoselectivity of Lipases and Esterases

Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering

Protein Engineering of Modular Polyketide Synthases

Cyanophycin Synthetases

Biosynthetic Pathway Engineering Strategies

Natural Polyester-Related Proteins: Structure, Function, Evolution and Engineering

Bioengineering of Sequence-Repetitive Polypeptides: Synthetic Routes to Protein-Based Materials of Novel Structure and Function

Silk Proteins - Biomaterials and Bioengineering