Microchip-Based Assay Systems explores recent progress in the microelectronics arena, the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world. While the area of microelectronics faces daunting challenges, the field also promises fascinating new advances that may translate into products such as sensors and devices that use nanometer-sized building blocks for real-world applications. The contributors to this volume are part of growing interdisciplinary efforts among pioneers in microelectronics, nanoscience, and health that will continue to create exciting new microchip-based applications across a variety of sectors. In the 16 chapters of this volume, leading scientists present technically precise microchip-based assays for a variety of applications. The protocols detailed in this volume will help foster interest in microchip-based assays by providing readers with all the tools necessary to create their own microchip-based assays targeted to new applications. Certainly, Microchip-Based Assay Systems will help to expand the scope and accelerate the transition of microchip-based assays from academic and industrial research and development centers to real-world use.
Written for:
Geneticists, biotechnologists, chemists, biochemists, molecular and cell biologists
Table of contents
Table of Contents / Microchip-based Assay Systems / Ed. Pierre N. Floriano
Microchip Electrophoresis for DNA Separation by Wire-Imprinted Microchannels on PMMA Substrates
Shu-Hui Chen
Fabrication of Porous Polymer Monoliths in Microfluidic Chips for Selective Nucleic Acid Concentration and Purification
Jay A. A. West, Brent C. Satterfield
Rapid Electrical Lysis of Bacterial Cells in a Microfluidic Device
Hsiang-Yu Wang, Padmapriya P. Banada, Dr. Arun Bhunia , Chang Lu
On-Chip Bioassay Using Immobilized Sensing Bacteria in Three-Dimensional Microfluidic Network
Hirofumi Tani, Koji Maehana, Tamio Kamidate
Microchip-based Enumeration of Human White Blood Cells
Pierre Floriano, Shelley Acosta, Nick Christodoulides, John T. McDevitt
Microchip for the Diagnosis of Cervical Cancer
Anja Gulliksen, Frank Karlsen
DNA Microchips Towards Molecular Signatures in Cervical Cancers
Yick F. Wong, Tony K.H. Chung, Vivian W. Wang, David I. Smith
Impedimetric-Detection for DNA Hybridization within Microfluidic Biochips
Louise Lingerfelt, James Karlinsey, James P. Landers, Anthony Guiseppi-Elie
Applications of Functional Protein Microarrays: Identifying Protein-Protein Interactions in an Array Format
Matthew A. Coleman, Peter T. Beernink, Julio A. Camarero, Joanna S Albala
A Microchip-based Assay for Interleukin-6
Nicolaos Christodoulides, Prya Dharsham, Jorge Wong, Pierre N. Floriano , Dean Neikirk , John T. McDevitt
Allergen Microarrays for the Diagnosis of Specific IgE Against Components of Cow milk and Hen Egg in a Multiplex Biochip-based Immunoassay
Christian Harwanegg, Sabine Hutter, Reinhard Hiller
Surface Plasmon Resonance Imaging on Polypyrrole Protein Chips: Application to Streptavidin Immobilization and Immunodetection
Emilie MERCEY, Ludivine GROSJEAN, Andre Roget, Thierry LIVACHE
Protein Array-based Multiplexed Cytokine Assays
Cheng C. Wang
Lectin Microarrays for Glycoprotein Analysis
Lara K. Mahal, Kanoelani Pilobello
Interaction of HIV RNA with Peptides Detected by Acoustic Shear Wave Sensor Operated in an On-line Format
Anil Deisingh, Satie Siewah , Nardos Tassew, Michael Thompson
Microchip-based Electrochemical Enzyme Immunoassays
Madhu Prakash Chatrathi, Greg Collins, Joseph Wang