Preface v
Contributors xi
1 A Review of Current and Future Molecular Diagnostic Tests
for Use in the Microbiology Laboratory
Geert Jannes and Daniel De Vos
2 Overview of DNA Purification for Nucleic Acid-based Diagnostics
From Environmental and Clinical Samples
Knut Rudi and Kjetill S. Jakobsen
3 Microarray-based Detection of Bacteria by On-chip PCR
Georg Mitterer and Wolfgang M. Schmidt
4 An Array Biosensor for Detection of Bacterial and Toxic
Contaminants of Foods
Miriam M. Ngundi and Chris R. Taitt
5 Detection of Neisseria meningitidis, Streptococcus pneumoniae
and Haemophilus influenzae in Blood and Cerebrospinal Fluid
Using Fluorescence-based PCR
Stuart C. Clarke
6 Use of Hybridization Probes in a Real-Time PCR Assay
on the LightCycler® for the Detection of MRSA
Andrea J. Grisold and Harald H. Kessler
7 Detection of Verotoxin Genes VT 1 and VT 2
in Escherichia coli O157:H7 in Minced Beef
Using Immunocpture and Real-time PCR
Justine Fitzmaurice
8 Application of a Quantitative Two-step qRT-PCR
to Bacterial Daignostics
Barry Glynn
9 Quencher Extension (QEXT) for Single Nucleotide Polymorphism Quantification in
Bacterial Typing and Microbial Community Analyses
Knut Rudi, Monika Zimonja and Beate Skøseng
10 Amplified Fragment Length Polymorphism and Protein Profiling
for Identification of Campylobacter lari Subgroups
Birgitta Duim and Jaap Wagenaar
11 Use of Peptide Nucleic Acid Probes for Rapid Detection
and Enumeration of viable Bacteria in Recreational Waters
and Beach Sand
Nwadiuto Esiobu
12 HOOF-Prints: Brucella Strain Typing by PCR Amplification
of Multilocus Tandem Repeat Polymorphisms
Betsy J. Bricker and Darla R. Ewalt
13 Detection of Legionella in Various Sample Types
Using Whole Cell Fluorescent In Situ Hybridization
Priscilla Declerck and Frans Ollevier
14 Identification of Diagnostic Proteins in Mycobacterium avium
subsp. paratuberculosis by Whole Genome Analysis Approach
John P. Bannantine and Michael L. Paustian
15 Diagnosois of Q Fever using Indirect Microimmunifluorescence
Guenievre Imbert and Bernard La Scola
16 Use of Macrophage Cell Cultures for Rapid Isolation of Intracellular Bacteria:
Mycobacterium bovis Model
Massimo Amadori, Marco Ritelli, Silvia Tagliabue,
nd Maria L. Pacciarini
Index