Branch	Locations	Status	Reservations	Due date	Barcode	Floor plan	Lending note
Branch: Dauerleihe	Locations: BI-30 6c (3.Ex.)	Status: borrowed	Reservations: 0	Due date: 1/1/2199	Barcode: 00270911	Floor plans: Floor plan	Lending note:
Branch: Hauptstelle	Locations: BI-30 6c (2.Ex.)	Status: available	Reservations: 0	Due date:	Barcode: 00268445	Floor plans: Floor plan	Lending note:

This fourth edition of “The Mouse Manual” is once again the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Its preeminent authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy have reorganized and updated this edition to include new information and protocols on:

 

generation of induced pluripotent stem cells

RNA microinjections

lentiviral microinjections and infection

assisted reproduction techniques for sperm and embryo cryopreservation

isolation, generation, and transplantation of spermatogonial stem cell lines

in utero electroporation of gene constructs into postimplantation embryos

vibratome sectioning of live and fixed tissues for imaging thick tissue sections

whole-mount fluorescent staining methods for three-dimensional visualization

Recombinant DNA techniques and methods for studies of mouse embryonic development have been updated from previous editions, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of this classic work appeared more than 20 years ago, with authors that included Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy. The field’s technological sophistication has grown exponentially but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.