Branch	Locations	Status	Reservations	Due date	Barcode	Floor plan	Lending note
Branch: Dauerleihe	Locations: VI-50 15	Status: borrowed	Reservations: 0	Due date: 12/7/2010	Barcode: 00182486	Floor plans: Floor plan	Lending note:

Contents

Acknowledgments

How To Use This Manual

PART I: AN OVERVIEW OF BACULOVIRUSES

1. Virus Structure and the Infection Process

2. Gene Organization, Regulation, and Function

3. Virus-Host Interactions

4. Summary of Baculovirus Features Relevant to Expression Factors

PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS

5. Choice of Virus and Host Species

6. Choice of Transfer Plasmid

7. Available Transfer Plasmids

8. Choosing a Parent Virus for Use in Vector Construction

9. Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System

PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION

10. Safety Considerations

11. Insect Cell Culture

12. Virus Methods

13. Contransfection and Recombinant Virus Identification

14. Characterizing Recombinant Gene Expression

15. Post-Translational Modification

PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE

16. Scale-Up of Protein Production in a Stirred Bioreactor , David L. Clemm

17. Scale-Up of Protein Production in an Airlift Fermenter , Cheryl Isaac Murphy

18. Insect Rearing and Infection

Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region

Appendix 2: Nucleotide Sequence of AcMNPV p10 Region

Appendix 3: List of Genes Expressed in the Baculovirus Expression System

Appendix 4: Suppliers

Appendix 5: Stock Solutions

Appendix 6: Excel Spreadsheet for TCIDnullnull Calculation

References

Index