About this book
This book presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities of using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general.
Table of contents
Table of Contents
1. Quantitative, Multiplexed Detection of Salmonella and Other
Pathogens by
Luminex
Sherry A. Dunbar and James W. Jacobsen
2. Detection of Salmonella by Bacteriophage Felix 01
Jonathan C. Kuhn
3. Physical Mapping of Salmonella Genomes
Shu-Lin Liu
4. Cloth-based Hybridization Array System for the Identification of
Antibiotic
Resistance Genes in Salmonella
Burton W. Blais and Martine Gauthier
5. Genome-based Identification of Molecular Analyses of Pathogenicity
Islands and
Genomic Islands in Salmonella enterica
Michael Hensel
6. Determination of the Gene Content of Salmonella Genomes by Microarray
Analysis
Steffen Porwollik and Michael McClelland
7. In-Vivo Excision, Cloning, and Broad-Host-Range Transfer of Large
Bacterial
DNA Segments Using VEX-Capture
James A. Wilson and Cheryl A. Nickerson
8. Amplified Fragment Length Polymorphism Analysis of Salmonella
enterica
Ruiting Lan and Peter R. Reeves
9. Salmonella Phages and Prophages - Genomics and Practical Aspects
Andrew M. Kropinski, Alexander Sulakvelidze, Paulina Konczy, and
Cornelius
Poppe
10. Salmonella Typhimurium Phage Typing for Pathogens
Wolfgang Rabsch
11. Salmonella Phages Examined in the Electron Microscope
Hans-W. Ackermann
12. Applications of Cell Imaging in Salmonella Research
Charlotte A. Perrett and Mark A. Jepson
13. Analysis of Kinesin Accumulation on Salmonella-containing Vacuoles
Audrey Dumont, Nina Schroeder, Jean-Pierre Gorvel, and Stéphane
Méresse
14. Magnesium, Manganese and Divalent Cation Transport Assays in Intact
Cells
Michael E. Maguire
15. Methods in Cell-to-Cell Signaling in Salmonella
Brian M. M. Ahmer, Jenee N. Smith, Jessica L. Dyszel, and AmberLindsay
16. Development of Salmonella Strains as Cancer Therapy Agents and
Testing in
Tumor Cell Lines
Abraham Eisenstark, Robert A. Kazmierczak, Alison Fea, Rula Khreis,
Dustin
Newman, and Heide Schatten
17. Further Resources for Molecular Protocols in Salmonella Research
Abraham Eisenstark and Kelly K. Edwards