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About this book

 

This book presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities of using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general.

 

 

Table of contents

 

Table of Contents

 

1. Quantitative, Multiplexed Detection of Salmonella and Other

Pathogens by

Luminex

Sherry A. Dunbar and James W. Jacobsen

 

2. Detection of Salmonella by Bacteriophage Felix 01

Jonathan C. Kuhn

 

3. Physical Mapping of Salmonella Genomes

Shu-Lin Liu

 

4. Cloth-based Hybridization Array System for the Identification of

Antibiotic

Resistance Genes in Salmonella

Burton W. Blais and Martine Gauthier

 

5. Genome-based Identification of Molecular Analyses of Pathogenicity

Islands and

Genomic Islands in Salmonella enterica

Michael Hensel

 

6. Determination of the Gene Content of Salmonella Genomes by Microarray

Analysis

Steffen Porwollik and Michael McClelland

 

7. In-Vivo Excision, Cloning, and Broad-Host-Range Transfer of Large

Bacterial

DNA Segments Using VEX-Capture

James A. Wilson and Cheryl A. Nickerson

 

8. Amplified Fragment Length Polymorphism Analysis of Salmonella

enterica

Ruiting Lan and Peter R. Reeves

 

9. Salmonella Phages and Prophages - Genomics and Practical Aspects

Andrew M. Kropinski, Alexander Sulakvelidze, Paulina Konczy, and

Cornelius

Poppe

 

10. Salmonella Typhimurium Phage Typing for Pathogens

Wolfgang Rabsch

 

11. Salmonella Phages Examined in the Electron Microscope

Hans-W. Ackermann

 

12. Applications of Cell Imaging in Salmonella Research

Charlotte A. Perrett and Mark A. Jepson

 

13. Analysis of Kinesin Accumulation on Salmonella-containing Vacuoles

Audrey Dumont, Nina Schroeder, Jean-Pierre Gorvel, and Stéphane

Méresse

 

14. Magnesium, Manganese and Divalent Cation Transport Assays in Intact

Cells

Michael E. Maguire

 

15. Methods in Cell-to-Cell Signaling in Salmonella

Brian M. M. Ahmer, Jenee N. Smith, Jessica L. Dyszel, and AmberLindsay

 

16. Development of Salmonella Strains as Cancer Therapy Agents and

Testing in

Tumor Cell Lines

Abraham Eisenstark, Robert A. Kazmierczak, Alison Fea, Rula Khreis,

Dustin

Newman, and Heide Schatten

 

17. Further Resources for Molecular Protocols in Salmonella Research

Abraham Eisenstark and Kelly K. Edwards