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This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible.

This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

 

Aus dem Inhalt

Animal Cell Culture - Essential Methods

 

John Davis (Editor)

 

1. The Cell Culture Laboratory

Susan Clarke and Janette Dillon

 

2. Sterilization

Peter Roberts

 

3. The Microscopy of Living Cells

Colin Gray and Daniel Zicha

 

4. Basic Techniques and Media, the Maintenance of Cell Lines, and Safety

John Davis

 

5. Serum-Free Culture Media

David Jayme and Stephen Gorfien

 

6. Cryopreservation and Cell Banking

Glyn N. Stacey, J. Ross Hawkins and Roland A. Fleck

 

7. Primary Culture of Specific Cell Types, and the Establishment of Cell Lines

Kee Woei Ng, Jan-Thorsten Schantz, and Chris Morris

 

8. Cloning

John Clarke, Alison Porter and John Davis

 

9. The Quality Control of Cell Lines and the Prevention, Detection and Cure of Contamination

Peter Thraves and Cathy Rowe

 

10. Systems for Culture Scale-Up

Jennifer Halsall and John Davis

 

11. Good Laboratory Practice (GLP) in the Cell Culture Laboratory

Barbara Orton