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Über dieses Buch

 

Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Chomosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.

Geschrieben für:

Geneticists, molecular and cellular biologists

Schlagworte:

 

* Eukaryotic organisms

* Gene disruption

* Gene knockouts

* Gene therapy

* Homologous recombination

* Prokaryotic organisms

 

 

Table of contents

 

Contents

 

Preface

 

Contributors

 

Biallelic Gene Knockouts in Chinese Hamster Ovary Cells

 

Naoko Yamane-Ohnuki, Kazuya Yamano, and Mitsuo Satoh

 

Highly Proficient Gene Targeting by Homologous Recombination in the Human pre-B Cell Line Nalm-6

 

Noritaka Adachi, Aya Kurosawa, and Hideki Koyama

 

Robust Cell Line Development Using Meganucleases

 

Jean-Pierre Cabaniols and Frédéric Pâques

 

Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells

 

Matthew Porteus

 

Gene Targeting in Drosophila and C. elegans with Zinc-finger Nucleases

 

Dana Carroll, Kelly J. Beumer, J. Jason Morton, Ana Bozas and Jonathan K. Trautman

 

Orpheus Recombination: A Comprehensive Bacteriophage System for Murine Targeting Vector Construction by Transplacement

 

Knut Woltjen, Kenichi Ito, Teruhisa Tsuzuki, and Derrick E. Rancourt

 

Transposon Mediated Mutagenesis in Somatic Cells: Identification of transposon-genomic DNA junctions

 

David A. Largaespada and Lara S. Collier

 

Insertional Mutagenesis of the Mouse Germline with Sleeping Beauty Transposition

 

Junji Takeda, Zsuzsanna Izsvák, and Zoltán Ivics

 

Conditional Gene Trapping Using the FLEx System

 

Thomas Floss and Frank Schnütgen

 

Steps Towards Targeted Insertional Mutagenesis with Class II Transposable Elements

 

Sareina Chiung-Yuan Wu, Kommineni J. Maragathavally, Craig J. Coates and Joseph M. Kaminski

 

Targeting integration of the Saccharomyces Ty5 retrotransposon

 

Troy L. Brady, Clarice L. Schmidt and Daniel F. Voytas

 

Site-Specific Chromosomal Integration Mediated by f C31 Integrase

 

Annahita Keravala and Michele P. Calos

 

Triplex-Mediated Gene Modification

 

Erica B. Schleifman, Joanna Y. Chin, and Peter M. Glazer

 

Allelic exchange of unmarked mutations in Mycobacterium tuberculosis.

 

Martin S. Pavelka, Jr.

 

Mycobacterial Recombineering

 

Julia C. van Kessel and Graham F. Hatfull

 

Chromosomal Engineering of Clostridium perfringens Using Group II Introns

 

Phalguni Gupta and Yue Chen