Over the past decade, tissue engineering has spread in importance in fields as distinct as biotechnology, biopharmaceutical manufacturing, chemical engineering, cell biology, developmental biology, gene therapy, medical sciences and organic chemistry. In Tissue Engineering, 2nd Edition, a team of experts from these various disciplines share recent advances in tissue engineering-related methodologies in order to provide a comprehensive volume that integrates a wide spectrum of methods. Written in the format of the highly successful Methods in Molecular Medicine series, each chapter seeks to drive tissue engineering into a clinical reality with step-by-step laboratory protocols, lists of necessary materials, and tips on troubleshooting and avoiding known pitfalls.
Thorough and timely, Tissue Engineering, 2nd Edition is an invaluable resource for all scientists leading the way toward the first successful implantation of a fully functional artificial organ.
Table of contents
Contents
Contributors
In Vitro Expansion of Tissue Cells by Conditional Proliferation
Tobias May, Hansjörg Hauser, and Dagmar Wirth
Stem Cell Engineering using Transducible Cre Recombinase
Lars Nolden, Frank Edenhofer, Michael Peitz, and Oliver Brüstle
Human Embryonic Stem Cells for Tissue Engineering
Daniel Kitsberg
Culture and Characterisation of Human Bone Marrow Mesenchymal Stem Cells
Bruno Delorme and Pierre Charbord
Skeletal ("Mesenchymal") Stem Cells for Tissue Engineering
Pamela Gehron Robey, Sergei A. Kuznetsov, Mara Riminucci,
and Paolo Bianco
Biomaterials/Scaffolds
Design of Bioactive, Multiphasic PCL/collagen Type I and Type II -
PCL-TCP/collagen Composite Scaffolds for Functional Tissue Engineering
of Osteochondral Repair Tissue by Using Electrospinning and FDM
Techniques
Detlef Schumann, Andrew K. Ekaputra, Christopher X.F. Lam, and Dietmar
W. Hutmacher
Synthetic Hydrogel Matrices for Guided Bladder Tissue Regeneration
Catharina A.M. Adelöw, and Peter Frey
Generation of Multicellular Tumour Spheroids by the Hanging Drop Method
Nicholas E. Timmins and Lars K. Nielsen
In-vitro Vascularization of Human Connective Microtissues
Jens M. Kelm, Wolfgang Moritz, Doerthe Schmidt, Simon P. Hoerstrup, and
Martin Fussenegger
Artificial Skin
Matthias Föhn and Holger Bannasch
Small Blood Vessel Engineering
Patrick Au, Josh Tam, Dai Fukumura, and Rakesh K Jain
Artificial Pancreas to Treat Type 1 Diabetes Mellitus
Riccardo Calafiore and Giuseppe Basta
Human Articular Chondrocytes Culture
Andrea Barbero and Ivan Martin
Cardiomyocytes from Human and Mouse Embryonic Stem Cells
Christine Mummery, Marcel A.G. van der Heyden, Teun P. de Boer, Robert
Passier, Dorien Ward, Stieneke van den Brink, Marga van Rooijen, Anja
van de Stolpe
Myocardial Restoration and Tissue Engineering of Heart Structures
Theo Kofidis, Knut Müller-Stahl, and Axel Haverich
Practical Aspects of Cardiac Tissue Engineering with Electrical
Stimulation
Christopher Cannizzaro, Nina Tandon, Elisa Figallo, Hyoungshin Park,
Sharon Gerecht, Milica Radisic, Nicola Elvassore, and Gordana
Vunjak-Novakovic
Biological Scaffolds for Heart Valve Tissue Engineering
Artur Lichtenberg, Serghei Cebotari, Igor Tudorache, Andres Hilfiker,
and Axel Haverich
In Vitro Heart Valve Tissue Engineering
Dörthe Schmidt, Anita Mol, Jens M. Kelm, and Simon P. Hoerstrup