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Volume 41 of Methods in Microbiology is a methods book designed to highlight procedures that will revitalize the purposes and practices of prokaryotic systematics.

This volume will notably show that genomics and computational biology are pivotal to the new direction of travel and will emphasise that new developments need to be built upon historical good practices, notably the continued use of the nomenclatural type concept and the requirement to deposit type strains in at least two service culture collections in different countries.

TOC:

The Need for Change: Embracing the Genome

William B. Whitman

An Introduction to Phylogenetics and The Tree of Life1

Tom A. Williams and Sarah E. Heap

The All-Species Living Tree Project

Pablo Yarza and Raul Munoz

16S rRNA Gene-based Identification of Bacteria and Archaea using the EzTaxon Server

Mincheol Kim and Jongsik Chun

Revolutionising Prokaryotic Systematics Through Next-Generation Sequencing

Vartul Sangal, Leena Nieminen, Nicholas P. Tucker and Paul A. Hoskisson

Whole Genome Analyses: Average Nucleotide Identity

David R. Arahal

Whole-Genome Sequencing for Rapid and Accurate Identification of Bacterial Transmission Pathways

Simon R. Harris and Chinyere K. Okoro

Identification of Conserved Indels that are useful for Classification and Evolutionary Studies

Radhey S. Gupta

Reconciliation Approaches to Determining HGT, Duplications, and Losses in Gene Trees

Olga K.Kamneva and Naomi L. Ward

Multi-locus Sequence Typing and the Gene-by-Gene Approach to Bacterial Classification and Analysis of Population Variation

Alison J. Cody, Julia S. Bennett and Martin C.J. Maiden

Multilocus Sequence Analysis: Bringing Bacterial Systematics to the Next Level

Xiaoying Rong and Ying Huang

Bacterial Typing and Identification by Genomic Analysis of 16s-23s rRNA Intergenic Spacer (ITS) Sequences

Volker Gärtler, Gangavarapu Subrahmanyam, Malathi Shakar, Biswajit Maiti and Indrani Karunasagar

MALDI-TOF Mass Spectrometry Applied to Classification and Identification of Bacteria

Peter Schumann and Thomas Maier

Continuing Importance of the â??Phenotypeâ? in the Genomic Era

Peter Kämpfer