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Branch: Hauptstelle	Locations: BC-90 50 (2)	Status: available	Reservations: 0	Due date:	Barcode: 00183733	Floor plans: Floor plan	Lending note:

Description

 

Like its predecessor Lab Ref, Volume 1, this book is a handy benchtop source of recipes and information needed for common laboratory protocols. Lab Ref, Volume 2 contains recipes for procedures as diverse as RNA interference, imaging, proteomics, and quantitative nucleic acid analysis, and is organized by application for quick and easy reference. All recipes were derived from recent manuals published by Cold Spring Harbor Laboratory Press, and each recipe is cross-referenced to its manual of origin. Reference tables and charts are included. To meet the growing need for computational resources in the biology laboratory, Lab Ref, Volume 2 also has an expanded Web site section with sources for biological materials, databases for acquiring and annotating genomic and proteomic data, and links to downloadable software.

 

Contents

 

Introduction

SECTION 1: QUANTITATIVE NUCLEIC ACID ANALYSIS

Isolation of Total DNA

Isolation of Total RNA

Selection of Poly(A) RNA from Total

Isolation and Amplification of RNA from Single Cells and Small Amounts of Tissue

Hybridization Analysis

Preparation of Nucleic Acid (Target) for Array Hybridization Analysis

Preparation of Nucleic Acid (Probe) for Array Hybridization Analysis

Other Quantitative Methods

SECTION 2: RNAi

RNAi in C. elegans

RNAi in D. melanogaster

RNAi in Plants

RNAi in Mammalians

RNAi in Mouse Oocytes and Early Embryos

SECTION 3: IMAGING

Preparation of Embryos for Live Cell Imaging

Imaging Fixed Tissue

Preparation of Mice for Imaging

Intracellular Imaging

General Information for Imaging

SECTION 4: PROTEIN ANALYSIS/PROTEOMICS

Basic Methods in Protein Analysis for Proteomics

Extraction of Proteins

Advanced Protocols in Identifying ProteinProtein Interactions for Proteomics

SECTION 5: ARABIDOPSIS

Microscopy

Genetic Manipulation

Protein Extraction

Nuclear Extraction

Histochemistry of GUS Fusion

SECTION 6: MURINE

In Vitro Culturing of Embryonic Stem Cells

Genotyping

Immunohistochemistry of Whole-mount Embryos

Other Buffers and Solutions

SECTION 7: STORAGE AND SHIPMENT OF BIOLOGICAL SAMPLES

SECTION 8: USEFUL FACTS AND TABLES

A. Useful Information and Reference Tables

B. Nomenclature Guide

C. Useful World Wide Web Sites

Appendix

Index