Contents
Preface ...............................................................................................................
Contributors .......................................................................................................
1 Treatment of Tissue Sections for In Situ Hybridization
Gregory H. Tesch, Hui Y. Lan,
and David J. Nikolic-Paterson ............................................................
2 Probe Design
Peter Roche .............................................................................................
3 cRNA Probes: Comparison of Isotopic and Nonisotopic
Detection Methods
Teresa Bisucci, Tim D. Hewitson, and Ian A. Darby ..............................
4 Tyramide Signal Amplification for DNA and mRNA
In Situ Hybridization
Ernst J. M. Speel, Anton H. N. Hopman,
and Paul Komminoth ..........................................................................
5 Expression Analysis of Murine Genes Using In Situ Hybridization
With Radioactive and Non-Radioactively Labeled Probes
Anne Chotteau-Leli--- vre, Pascal Doll--- ,
and Fran---- oise Gofflot .........................................................................
6 Non-Radioactive In Situ Hybridization on Frozen Sections
and Whole Mounts
Julia Asp, Alexandra Abramsson, and Christer Betsholtz.......................
7 In Situ Hybridization of Whole Mount Embryos
Murray Hargrave, Josephine Bowles, and Peter Koopman ....................
8 In Situ Detection of Epstein-Barr Virus and Phenotype
Determination of EBV-Infected Cells
Gerald Niedobitek and Hermann Herbst ...............................................
9 PNA- Situ Hybridization Method for Detection of HIV-1
DNA in Virus-Infected Cells and Subsequent Detection
of Cellular and Viral Proteins
Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda ....................
10 Electron Microscopy and In Situ Hybridization: Expression
of P2Y2 Receptor mRNA in the Cerebellum
Andrzej Loesch and Rainer Glass ...........................................................
11 Free-Floating Sections of Brain In Situ
Neil Owens .............................................................................................
12 An In Situ Hybridization Technique to Detect Low-Abundance
Slug mRNA in Adherent Cultured Cells
Changsun Choi, Laurie G. Hudson, Pierre Savagner,
and Donna F. Kusewitt .......................................................................
13 Identification of Transplanted Human Cells
in Animal Tissues
Daniel Benten, Kang Cheng, and Sanjeev Gupta ...................................
14 In Situ Hybridisation to Plant Tissues and Chromosomes
Andreas Houben, Sharon J. Orford,
and Jeremy N. Timmis ........................................................................
15 Histochemical Localization of Cell Proliferation Using
In Situ Hybridization for Histone mRNA
Tim D. Hewitson, Kristen J. Kelynack,
and Ian A. Darby ................................................................................
16 Histochemical Localization of Apoptosis With In Situ
Labeling of Fragmented DNA
Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby ..............................
17 The Use of Combined Immunohistochemical Labeling
and In Situ Hybridization to Co-Localize mRNA and
Protein in Tissue Sections
Malcolm D. Smith, Michael Ahern, and Mark Coleman ........................
18 Semi-Quantitative In Situ Hybridization Using
Radioactive Probes to Study Gene Expression
in Motoneuron Populations
Paul D. Storer and Tracey DeLucia ........................................................
19 Quantitative In Situ Hybridization of Tissue Microarrays
Adrian M. Jubb, Thinh Q. Pham, Gretchen D. Frantz,
Frank V. Peale, and Kenneth J. Hillan ................................................