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Volume 1; Mass spectrometry for lipidomics

methods and applications
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Year: [2023]
Publisher: Weinheim, Germany, Wiley-VCH
Series: Mass spectrometry for Lipidomics
Media group: Ausleihbestand
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Branch: Hauptstelle Locations: SP-20 45 (1) Status: borrowed Reservations: 0 Due date: 5/15/2024 Barcode: 00317780 Floor plans: Floor plan Lending note:

Content

Cover -- Volume 1 -- Title Page -- Copyright Page -- Contents -- Preface -- Chapter 1 Introduction to Lipidomics -- 1.1 Preface -- 1.2 Historical Perspective -- 1.3 Sampling and Preanalytics -- 1.4 Reference Materials and Biological Reference Ranges -- 1.5 Clinical Lipidomics -- 1.6 Identification and Annotation -- 1.7 Quantitation -- 1.8 Lipid Ontology -- References -- Part I Analytical Methodologies in Lipidomics -- Chapter 2 Preanalytics for Lipidomics Analysis -- 2.1 Safety -- 2.2 Introduction -- 2.3 Sample Origin -- 2.4 Sample Collection -- 2.5 Tissue Homogenization -- 2.5.1 Mortar and Pestle -- 2.5.2 Rotor-Stator -- 2.5.3 Blender -- 2.5.4 Potter-Elvehjem -- 2.5.5 Bead Mill -- 2.6 Liquid-Liquid Extraction (LLE) -- 2.6.1 Folch Method -- 2.6.2 Bligh and Dyer (BD) Method -- 2.6.3 Modified Folch and Bligh/Dyer (BD) Methods -- 2.6.4 Rose and Oaklander (RO) Method -- 2.6.5 Matyash or Methyl-tert-Butyl Ether (MTBE) Method -- 2.6.6 BUME Method -- 2.6.7 Alshehry Method -- 2.6.8 Three-Phase Lipid Extraction (3PLE) -- 2.7 Resuspension and Solubilization -- 2.8 Automation -- 2.9 Tips and Tricks -- Acknowledgments -- References -- Chapter 3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry -- 3.1 Introduction -- 3.2 Complexity of Crude Lipid Extracts -- 3.2.1 Main Lipid Classes in Mammalian Samples -- 3.2.2 Bond Types as Structural Features -- 3.2.3 Fatty Acids as the Major Building Blocks -- 3.2.4 Lipid Species and Double-Bond Series -- 3.3 Introduction to Mass Spectrometry of Lipids -- 3.3.1 Annotation of Lipid Structures Analyzed by MS -- 3.3.2 Isomers -- 3.3.3 Isobars and the Type-II Isotopic Overlap -- 3.4 Overview of Direct Infusion MS Workflows -- 3.5 Sample Preparation -- 3.5.1 Preanalytics - Sample Stability -- 3.5.2 Lipid Extraction -- 3.5.3 Solvents, Additives, and Lipid Concentration -- 3.5.4 Sample Derivatization.

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Statement of Responsibility: edited by Michal Holcapek and Kim Ekroos
Year: [2023]
Publisher: Weinheim, Germany, Wiley-VCH
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Classification: Search for this systematic SP-20, AL-30
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ISBN: 9783527352210
ISBN (2nd): 3527350152
Description: [first edition], 2 Bände, illustrations
Series: Mass spectrometry for Lipidomics
Participating parties: Search for this character Holcapek, Michal (editor); Ekroos, Kim (editor)
Language: Englisch
Original title: Mass spectrometry for lipidomics
Footnote: [Dieses Werk gibt es inhaltsgleich auch in digitaler Form.]
Media group: Ausleihbestand