Contents
Preface v
Contributors xi
Part I Communities and Biofilms
1 Isolation and Molecular Characterization of Seawater Bacteria
Juliana M. Benito, Gustavo A. Lovrich, Faustino Siñeriz,
and Carlos M. Abate 3
2 Reverse Sample Genome Probing to Monitor Microbial Communities
E. Anne Greene and Gerrit Voordouw 11
3 T-RFLP Analysis: A Rapid Fingerprinting Method for Studying
Diversity, Structure, and Dynamics of Microbial Communities
Werner Liesack and Peter F. Dunfield 23
4 Assessing Bacterial DNA Content in Aquatic Systems
Garrett W. Perney, Betsy R. Robertson, and D. K. Button 39
5 Multiplexed Identification and Quantification of Analyte DNAs
in Environmental Samples Using Microspheres and Flow Cytometry
Mary Lowe, Alex Spiro, Anne O. Summers, and Joy Wireman 51
6 Molecular Characterization of Microbial Communities From Marine Environments
Nicolás G. Barengo, Juliana M. Benito, and Carlos M. Abate 75
7 Yeasts: Ecology in Northwest Argentina
Lucía I. C. de Figueroa, M. Alejandra Martínez,
and John F. T. Spencer 83
8 Aeromonads in Environmental Waters
Anavella Gaitan Herrera 97
9 Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in
Subsurface Ground Water
Mark E. Fuller 103
10 Bench Scale Flow Cell for Nondestructive Imaging of Biofilms
Eric S. Gilbert and Jay D. Keasling 109
PART II FERMENTED MILKS
11 Alpha-Galactosidase Assay in Fermented Soymilk Products
Marisa S. Garro, Graciela Font de Valdez,
and Graciela Savoy de Giori 121
12 Considerations to Avoid the Overestimation of Exopolysaccharides Produced by Lactic
Acid Bacteria
María Inés Torino and Graciela Font de Valdez 125
13 Determination of Oligosaccharides in Fermented Soymilk Products
by High Performance Liquid Chromatography
Marisa S. Garro, Graciela Font de Valdez,
and Graciela Savoy de Giori 135
14 Evaluation of Minimal Nutritional Requirements of Lactic Acid
Bacteria Used in Functional Foods
Elvira M. Hébert, Raúl R. Raya, and Graciela Savoy de Giori 139
PART III NUCLEIC ACIDS, RECOVERY, AND DETERMINATION
15 A Simple Method for Obtaining DNA Suitable for RAPD Analysis
From Azospirillum
Raúl O. Pedraza and Juan C. Díaz Ricci 151
16 PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of
Environmental Bacteria Isolates
M. Alejandra Martínez and Faustino Siñeriz 159
17 A DNA/DNA Hybridization Method for the Detection
and Quantification of Specific Bacterial Taxa in Natural Environments
Christopher E. Bagwell and Charles R. Lovell 169
18 13C and 1H NMR Study of the Glycogen Futile Cycle in Fibrobacter succinogenes
Anne-Marie Delort, Genevieve Gaudet and Evelyne Forano 175
19 Chemical Analysis and Biological Removal of Wood Lipids Forming Pitch Deposits in Paper
Pulp Manufacturing
Ana Gutiérrez, José C. del Río, and Ángel T. Martínez 191
20 Propolis: Chemical Micro-Heterogeneity and Bioactivity
José Domingos Fontana, Juliana Adelmann, Mauricio Passos,
Marcelo Maraschin, Cristina A. de Lacerda,
and Fernando Mauro Lanças 205
21 Enzymatic Saccharification of Cellulosic Materials
Luiz Pereira Ramos and José Domingos Fontana 221
22 Molecular Identification of Microbial Populations in Petroleum-Contaminated Groundwater
Kazuya Watanabe, Natsuko Hamamura, and Nobuo Kaku 237
23 Identification of Copper-Resistant Microorganisms by PCR
Virginia H. Albarracín, Juliana M. Benito, Manuel Siñeriz Louis,
María J. Amoroso, and Carlos M. Abate 245
24 Selection of Tolerant Heavy Metal Yeasts From Different Polluted Sites
Liliana Villegas, María J. Amoroso, and Lucía I. C. de Figueroa 251
25 Medium for Differential Enumeration of Lactobacillus casei
and Lactobacillus acidophilus From Lyophilized Mixed Cultures
Mónica M. Locascio, Rosalía Alesso, Vilma I. Morata,
and Silvia N. González 259
26 Silk as a Means of Recovering Bacteriocin From a Culture Medium
M. Carina Audisio and María C. Apella 265
27 Optimization of a Method for Isolating Plasmid DNA From Lactic
Acid Bacteria From Wine
Fabiana M. Saguir and María C. Manca de Nadra 271
28 Simultaneous and Sequential Methods to Study Interactions
Between Yeast and Lactic Acid Bacteria From Wine
Marta E. Farías and María C. Manca de Nadra 277
29 Method for Determining Lindane Concentration in Water and Solid Samples
Claudia S. Benimeli, Adriana P. Chaile, and María J. Amoroso 281
30 Extracellular Hydrolytic Enzymes Produced by Yeasts
Fabio Vazquez, Martha Dina Vallejo Herrera,
Lucía I. C. de Figueroa, and María Eugenia Toro 285
31 Extracellular Hydrolytic Enzymes Produced by Phytopathogenic
Fungi
Martha Dina Vallejo Herrera, María Eugenia Toro,
Lucía I. C. de Figueroa, and Fabio Vazquez 301
32 An Analysis of Microorganisms in Environments Using Denaturing Gradient Gel
Electrophoresis
Paul W. Baker and Shigeaki Harayama 325
33 Testing for Evolutionary Correlations in Microbiology Using Phylogenetic Generalized
Least Squares
Scott R. Miller 341
34 Conjugated Linoleic Acid Detection Produced by Dairy Starter
Cultures
Carina Van Nieuwenhove, Silvia González de Elías,
and Alicia Bardón 355
PART IV REVIEWS
35 Techniques for Manipulating the Bacterial Endophyte Bacillus mojavensis
Charles W. Bacon and Dorothy M. Hinton 361
36 Engineering of Bacteria Using the Vitreoscilla Hemoglobin Gene
to Enhance Bioremediation of Aromatic Compounds
Benjamin C. Stark, Dale A. Webster, and Krishna R. Pagilla xxx
37 The Use of Chemical Shift Regents and 23Na NMR to Study Sodium Gradients in
Microorganisms
Anne-Marie Delort, Genevieve Gaudet, and Evelyne Forano xxx
38 Denaturing Gradient Gel Electrophoresis (DGGE) as a Fingerprinting Tool for Analyzing
Microbial Communities in Contaminated Environments
Laurent Eyers, Spiros N. Agathos, and Said El Fantroussi xxx
Index xxx