After thirteen years, the editors of Methods for General and Molecular Microbiology have prepared a new, state-of-the-art volume bringing this invaluable resource up to the present. This edition is much expanded with two entirely new sections on Community and Genomic Analysis and on Mycology (with emphasis on filamentous fungi) and 47 chapters compared to 31 in the previous edition.
This book will once again serve as a first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. Covering methods for microbes representing all three domains of life--Bacteria, Archaea, and Eukarya--this manual should be on the bookshelf of every serious practitioner of microbiology in academia, industry, and government, and in clinical laboratories. It will serve as a rich resource on methods for seasoned professionals as well as for students at all levels.
Many of the new chapters cover important topics such as molecular genetics of archaea; molecular genetic manipulations using phages as tools; state-of-the-art technology used in studying complex, naturally occurring microbial communities; metabolism of natural polymers including cellulose, hemicellulose, and lignin; DNA microarray technology; physiology, metabolism, molecular genetic approaches to the study of filamentous fungi, and general and molecular approaches for studying microbial symbioses.
Each section details reliable methods that are widely applicable to basic studies. Additionally, chapters present background principles to facilitate understanding of the how and why of a given method, followed by a step-by-step description of the procedure. Common problems, precautions, and pitfalls of the methods are also presented. In many cases, commercial sources for equipment and materials are also given.
Table of Contents
I. Morphology and Ultrastructure
Introduction to Morphology and Ultrastructure, T. J. Beveridge, Editor
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1. Light Microscopy, R. G. E. Murray and Carl F. Robinow
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2. Sampling and Staining for Light Microscopy, Terry J. Beveridge, J. R. Lawrence, and Robert G. E. Murray
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3. Laser Scanning Microscopy, John R. Lawrence and T. R. Neu
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4. Electron Microscopy, Terry J. Beveridge, Dianne Moyles, and Bob Harris
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5. Computational Image Analysis and Reconstruction from Transmission Electron Micrographs, George Harauz
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6. Atomic Force Microscopy, Yves F. Dufrêne
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7. Cell Fractionation, Susan F. Koval and G. Dennis Sprott
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8. Antigen-Antibody Reactions, Lucy M. Mutharia and Joseph S. Lam
II. Growth
Introduction to Growth, John A. Breznak, Editor
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9. Growth Measurement, Arthur L. Koch
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10. Nutrition and Media, David Emerson, Jane Tang, Rosali J. Cote, and Robert L. Gherna
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11. Enrichment and Isolation, Andreas Teske, Herbert Cypionka, John G. Holt, and Noel R. Krieg
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12. Culture Techniques, Syed A. Hashsham
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13. Energetics, Stoichiometry, and Kinetics of Microbial Growth, Syed A. Hashsham and Sam W. Bauschke
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14. Physicochemical Factors in Growth, John A. Breznak and Ralph N. Costilow
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15. Phenotypic Characterization and the Principles of Comparative Systematics, Brian J. Tindall, Johannes Sikorski, Robert A. Smibert, and Noel R. Krieg
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16. General Methods to Investigate Microbial Symbioses, Todd A. Ciche and Shana K. Goffredi
III. Metabolism
Introduction to Metabolism, C. A. Reddy, Editor
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17. Physical Analysis and Purification Methods, Scott B. Mulrooney, Willis A. Wood, and J. Robert Paterek
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18. Chemical Analysis, Lacy Daniels, Richard S. Hanson, and Jane A. Phillips
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19. Enzymatic Activity, Robert P. Hausinger and Allen T. Phillips
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20. Permeability and Transport, Robert E. Marquis
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21. Bacterial Respiration, Robert P. Gunsalus, Gary Cecchini, and Imke Schröder
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22. Carbohydrate Fermentations, R. Meganathan, Yamini Ranganathan, and C. A. Reddy
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23. Metabolism of Aromatic Compounds, Jerome J. Kukor, Boris Wawrik, and Gerben J. Zylstra
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24. Cellulases, Hemicellulases, and Pectinases, Michael E. Himmel, John O. Baker, William S. Adney, and Stephen R. Decker
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25. Lignin and Lignin-Modifying Enzymes, Carlos G. Dosoretz and C. A. Reddy
IV. Molecular Genetics
Introduction to Molecular Genetics, C. A. Reddy, Editor
L. R. Snyder, Co-Editor
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26. Similarity Analysis of DNAs, John L. Johnson and William B. Whitman
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27. Nucleic Acid Analysis, William Hendrickson and Donald Walthers
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28. Measuring Spontaneous Mutation, Patricia L. Foster
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29. Transposon Mutagenesis, Silvia Rossbach and Frans J. de Bruijn
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30. Plasmids, Marcelo E. Tolmasky, Luis A. Actis, Timothy J. Welch, and Jorge H. Crosa
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31. Gene Transfer in Gram-Negative Bacteria, Joseph E. Peters
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32. Gene Exchange in Gram-Positive Bacteria, Christopher J. Kristich, Christine E. Salomon, and Gary M. Dunny
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33. Genetics of Archaea, Kevin R. Sowers, Paul H. Blum, and Shiladitya DasSarma
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34. Genetic Manipulations Using Phages, Graham F. Hatfull, Debbie Jacobs-Sera, Michelle H. Larsen, and William R. Jacobs, Jr.
V. Community and Genomic Analysis
Introduction to Community and Genomic Analysis, Thomas M. Schmidt, Editor
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35. Characterization of Bacterial Genome Sequences by Similarity Searching, William R. Pearson
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36. Reconstructing and Interpreting Evolutionary Relationships, Christophe J. Douady and Camilla L. Nesbø
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37. Microbial Nucleotide Fingerprints in Nature, David M. Karl
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38. Construction of BAC and Fosmid Libraries from Naturally Occurring Microbial Populations, Edward F. DeLong
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39. Single Cell Identification by Fluorescence In Situ Hybridization, Bernhard M. Fuchs, Jakob Pernthaler, and Rudolf Amann
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40. Measurement of rRNA Abundance by Hybridization with Oligodeoxynucleotide Probes, Daniel H. Buckley and Thomas M. Schmidt
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41. Analysis of Microbial Communities with Denaturing Gradient Gel Electrophoresis and Terminal Restriction Fragment Length Polymorphism, Terence L. Marsh and Cindy H. Nakatsu
VI. Mycology
Introduction to Mycology, George A. Marzluf, Editor
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42. Methods for Studying Terrestrial Fungal Ecology and Diversity, R. G. Thorn, J. Scott, and M. A. LaChance
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43. Physiology, Metabolism, and Molecular Aspects of Filamentous Fungi, George A. Marzluf
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44. Microbiological and Genetic Methods for Filamentous Fungi, Rowland H. Davis and A. John Clutterbuck
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45. Principles and Practice of DNA Microarray Technology, Krishnamurthy Natarajan, Matthew J. Marton, and Alan G. Hinnebusch
APPENDICES:
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46. Laboratory Safety, W. Emmett Barkley and C. A. Mickelson
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47. Culture Preservation, Robert L. Gherna and C. A. Reddy