Table of Contents
Protein Kinases in Drug Discovery: Rationale, Success, and Challenge; Dana L. Johnson and Jan L. Sechler
An Introduction to the Protein Tyrosine Phosphatase Gene Family and Screening Assay Development; Dominique Perrin and Rob Hooft van Huijsduijnen
Time-Resolved Fluorescence Based Assays for Kinases; Zhuyin Li and Tina Garyantes
Development of High-throughput Screening Assays in Scintillating Microplates (FlashPlates) to Identify Inhibitors of Kinase Activity; Stewart Emanual
Development of High-Throughput Screening Assays for Kinase Drug Targets using AlphaScreen Technology; D. Wenham, C. Illy, J.A. St. Pierre, and N. Bouchard
Homogeneous High-Throughput Screening (HTS) Assays for Serine/Threonine Kinases Using ß-Galactosidase Enzyme Fragment Complementation; I. Vainshtein, T. Naqvi, A. Lim, R. Rouhani, L. Kauffman, R. Singh, and R.M. Eglen
IMAP Assays for Assaying Protein Kinases; R. Sportsman
A Homogenous, Luminescent, High-Throughput, Versatile Assay for a Wide Range of Kinases; S.A. Goueli, K. Hsiao, and B. Bulleit
Proteases as Drug Targets; R.L. Thurmond and J.P. Edwards
A Comparison of Homogeneous Bioluminescent and Fluorescent Methods for Protease Assays; M. O'Brien
Scintillation Proximity Assay (SPA) Receptor Binding Assays; J.R. Cook, R. Graves, K. Lowitz, M.J. Price-Jones, J.A. Berry and K.T. Hughes
Radioligand Binding Filtration Assay: Full Automation; S.K.F. Wong
Nuclear Receptors As Drug Targets; P.D. Pelton
Nuclear Receptor Scintillation Proximity Assays; D. Powell and M.J. Price-Jones
Homogenous Assay Development for Nuclear Receptor Using the AlphaScreen Technology; N. Rouleau and R. Bossé
Development of Nuclear Receptor Homogenous Assay Using the Lance Technology; N. Rouleau, P. Hurskainen, I. Hemmilä and R. Bossé
The Emerging Role of Cell-based Assays in Discovery; R. Garippa
The Preparation of Cells for High-Content Screening; A. Hoffman
The Evolution of cAMP Assays; P. Kasila and H. Harney
A Homogenous Fluorescent Polariaiton assay for Inositol 1,4,5-Trisphoshpate (Ins P3); P. Fung, R. Singh, L. Kauffman, R.M. Eglen and T. Naqvi
Scintillation Proximity Assay of Inositol Phosphates; W. Zheng and P. Brandish
Measuring Calcium Mobilization with Gq-Coupled GPCRs Using the Fluorometric Imaging Plate Reader (FLIPR); J. Dunlop, Y. Zhang, R. Ring and D. Kowal
Development of FLIPR-Based HTS Assay for Gi-Coupled GPCRs; C. Chen, C. Smith, L. Minor, and B. Damiano
Aurora Assays; P, Kunapuli
Membrane Potential Based Assays for Ion Channels and Electrogenic Transporters; Q, Lü, S, Lin, and J, Dunlop
Reporter Gene Assays for Drug Discovery; K.A. Houck, W.P. Bocchinfuso, M.S. Dowless, and K.M. Borchert
mRNA Detection from Cells Using Quantigene® Branched DNA Technology; L. Minor
Homogeneous Miltiwell Assays for Measuring Cell Viability, Cytotoxicity and Apoptosis; Terry Riss, R.A. Moravec, M.A. O'Brien, E.M. Hawkins, and A. Niles
Development of Image-Bases Assays for Drug Discovery; S.M. Catalano
GPCR Internalization Measured by Image Analysis; R. Graves, M.J. Francis, L. Smith, J.R. Cook, and E.J. Adie
TRANSFLUOR®, a Universal Cell-Based Assay for Screening G-Protein Coupled Receptors; R. Oakley C.L. Cowan, C.C. Hudson, and C.R. Loomis