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High-Throughput RNAi screening

methods and protocols
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Year: [2016]
Publisher: New York, NY, Humana Press
Series: Methods in molecular biology; 1470 , Springer protocols
Media group: Ausleihbestand
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Branch: Hauptstelle Locations: GE-40 318 Status: available Reservations: 0 Due date: Barcode: 00297992 Floor plans: Floor plan Lending note:

Content

High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In High-Throughput RNAi Screening: Methods and Protocols, expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, High-Throughput RNAi Screening: Methods and Protocols provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research.
 

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Statement of Responsibility: editors David O. Azorsa, Institute of Molecular Medicine, Phoenix Children's Hospital, Phoenix, Arizona, USA, Shilpi Arora, Constellation Pharmaceuticals, Cambridge, Massachuetts, USA
Year: [2016]
Publisher: New York, NY, Humana Press
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Classification: Search for this systematic GE-40, GE-30
Subject type: Search for this subject type Laborhandbuch
ISBN: 978-1-4939-6335-5
ISBN (2nd): 1-4939-6335-X
Description: xi, 262 Seiten : Illustrationen, Diagramme
Series: Methods in molecular biology; 1470 , Springer protocols
Tags: Genetik Methoden; Molekulare Genetik
Participating parties: Search for this character Azorsa, David O. [Herausgeber]; Arora, Shilpi [Herausgeber]
Language: Englisch
Media group: Ausleihbestand