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Mass spectrometry data analysis in proteomics

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Year: 2007
Publisher: Totowa, NJ, Humana Press
Series: Methods in molecular biology; 367
Media group: Ausleihbestand
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Branch: Hauptstelle Locations: NA-30 153 Status: available Reservations: 0 Due date: Barcode: 00183601 Floor plans: Floor plan Lending note:

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About this book
 
This is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. As MS is a high throughput technique, proteomic researchers must attend carefully to the associated field of data analysis, and this volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters.
 
Several different types of MS data are discussed, followed by a description of optimal methods for conversion of raw data into peak lists for input to search engines. Choosing the most accurate and complete databases is emphasized, and a report of available sequence databases is included. Methods for assembling expressed sequence tags (ESTs) into assembled nonredundant databases are provided, along with protocols for further processing the sequences into a format suitable for MS data. This book describes publicly available applications whenever possible.

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Statement of Responsibility: edited by Rune Matthiesen
Year: 2007
Publisher: Totowa, NJ, Humana Press
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Classification: Search for this systematic NA-30, SP-20
Subject type: Search for this subject type Methodenbuch
ISBN: 9781588295637
ISBN (2nd): 1-58829-563-X
Description: X, 320 S. : Ill., graph. Darst.
Series: Methods in molecular biology; 367
Tags: Mass spectrometry; LC; Proteomics; Methods; NLM; Spectrum analysis, Mass; Naturstoffe; Proteine; Massenspektroskopie; Methodology; Expressed sequence tags
Participating parties: Search for this character Matthiesen, Rune [Hrsg.]
Language: englisch||
Footnote: Literaturangaben
Media group: Ausleihbestand