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Bacterial transcriptional control

methods and protocols
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Year: 2015
Publisher: New York, NY, Humana Press
Series: Methods in molecular biology; 1276 , Springer protocols
Media group: Ausleihbestand
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Branch: Hauptstelle Locations: MB-20 105 Status: available Reservations: 0 Due date: Barcode: 00271047 Floor plans: Floor plan Lending note:

Content

This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls.
 
Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future.
 

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Statement of Responsibility: edited by Irina Artsimovitch ; Thomas J. Santangelo
Year: 2015
Publisher: New York, NY, Humana Press
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Classification: Search for this systematic MB-20, GE-30, GE-40
Subject type: Search for this subject type Laborhandbuch
ISBN: 978-1-493-92391-5
ISBN (2nd): 1-493-92391-9
Description: XI, 342 S. : Ill., graph. Darst.
Series: Methods in molecular biology; 1276 , Springer protocols
Tags: Bakterien / Allgemein und Morphologie; Molekulare Genetik; Genetik Methoden
Participating parties: Search for this character Artsimovitch, Irina [Hrsg.]; Santangelo, Thomas J. [Hrsg.]
Language: Englisch
Footnote: Literaturangaben
Media group: Ausleihbestand