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In miRNomics: MicroRNA Biology and Computational Analysis, expert researchers in the field present an overview of the current state of the art and aim to put the respective areas of research into a larger perspective. These include methods and techniques ranging from miRNA biogenesis, their biological function, computational analyses to their medical implications and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, miRNomics: MicroRNA Biology and Computational Analysis seeks to aid scientists in the further study into miRNA research and statistics.

 

TABLE OF CONTENTS

1. Introduction to MicroRNAs in Biological Systems

 

Ay?e Elif Erson-Bensan

 

2. The Role of MicroRNA's in Biological Processes

 

Kemal U?ur Tüfekci, Ralph Leo Johan Meuwissen, and ?ermin Genç

 

3. The Role of MicroRNAs in Human Diseases

 

Kemal U?ur Tüfekci, Meryem Gülfem Öner, Ralph Leo Johan Meuwissen, and ?ermin Genç

 

4. Introduction to Bioinformatics

 

Tolga Can

 

5. MicroRNA and Non-Coding RNA Related Date Sources

 

Patrizio Arrigo

 

6. High Throughput Approaches for MicroRNA Expression Analysis

 

Bala Gür Dedeo?lu

 

7. Introduction to Machine Learning

 

Yal?n Ba?tanlar and Mustafa Özuysal

 

8. Introduction to Statistical Methods for MicroRNA Analysis

 

Gökmen Zararsiz and Erdal Co?gun

 

9. Computational Bioinformatics Methods for MicroRNA Gene Prediction

 

Jens Allmer

 

10. Machine Learning Methods for MicroRNA Gene Prediction

 

Mü?erref Duygu Saçar and Jens Allmer

 

11. Functional, Structural, and Sequence Studies of MicroRNA

 

Chanchal K Mitra and Kalyani Korla

 

12. Computational Methods for MicroRNA Target Prediction

 

Hamid Hamzeiy, Jens Allmer, and Malik Yousef

 

13. MicroRNA Target and Gene Validation in Viruses and Bacteria Patrizio Arrigo and D Baroni

 

14. Gene Reporter Assay to Validate MicroRNA Targets in Drosophila S2 Cells

 

Bünyamin Akgül and Ça?da? Gökta?

 

15. Computational Prediction of MicroRNA Function and Activity Hasan O?ul

 

16. Analysis of MicroRNA Expression using Machine Learning

 

Henry Wirth, Mehmet Volkan Çak?r, Lydia Hopp, and Hans Binder

 

17. MicroRNA Expression Landscapes in Stem Cells, Tissues, and Cancer

 

Mehmet Volkan Çak?r, Henry Wirth, Lydia Hopp, and Hans Binder

 

18. Master Regulators of Post-Transcriptional Gene Expression are Subject to Regulation

 

Syed Muhammad Hamid and Bünyamin Akgül

 

19. Use of MicroRNAs in Personalized Medicine

 

Ç???r Biray Avc? and Yusuf Baran