NA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines. Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.
Contents:
Part I: DNA Vaccine Design and Enhancement
1. A Blueprint for DNA Vaccines Design
Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi
2. Enhancement of Plasmid-Mediated Transgene Expression
Daniela Fioretti, Sandra Iurescia, and Monica Rinaldi
3. Strategies for Improving DNA Vaccines Performance
Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi
4. Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies
Elisabete Borges Freitas, Ana Margarida Henriques, Miguel Fevereiro, Duarte Miguel Prazeres, and Gabriel Amaro Monteiro
5. Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies
Ronald J. Jackson, David B. Boyle, and Charani Ranasinghe
6. Development of Antibiotic-Free Selection System for Safer DNA Vaccination
Jeremy M. Luke, Aaron E. Carnes, and James A. Williams
Part II: Delivery System
7. Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin
Anita Gothelf and Julie Gehl
8. DNA Vaccination in Skin Enhanced by Electroporation
Kate E. Broderick, Amir S. Khan, and Niranjan S. Sardesai
9. Intradermal Vaccination by DNA Tattooing
Joost H. van den Berg, Koen Oosterhuis, Ton N.M. Schumacher, John B.A.G. Haanen, and Adriaan D. Bins
10. Microneedle Applications for DNA Vaccine Delivery to the Skin
Hae-yong Seok, Hyemee Suh, Sunghyun Baek, and Yeu-Chun Kim
11. Multivalent DNA-Based Vectors for DNA Vaccines Delivery
Young Hoon Roh, Kwang Lee, Jessica Jane Ye, and Dan Luo
12. Superparamagnetic Nanoparticles Delivery of DNA Vaccine
Fatin Nawwab Al-Deen, Cordelia Selomulya, Charles Ma, and Ross L. Coppel
Part III: Production, Purification, and Quality
13. Plasmid Fermentation Process for DNA Immunization Applications
Aaron E. Carnes and James A. Williams
14. Pharmaceutical Grade Large Scale Plasmid DNA Manufacturing Process
Marco Schmeer and Martin Schleef
Part IV: New Vaccine Applications
15. Protective and Therapeutic DNA Vaccination Against Allergic Diseases
Almedina Isakovic, Richard Weiss, Josef Thalhamer, and Sandra Scheiblhofer
16. Immunotherapy for Alzheimer’s Disease: DNA- and Protein-Based Epitope Vaccines
Hayk Davtyan, Irina Petrushina, and Anahit Ghochikyan
17. Tetravalent DNA Vaccine Product as a Vaccine Candidate Against Dengue
Kevin R. Porter, Nimfa Teneza-Mora, and Kanakatte Raviprakash
18. DNA Vaccination as a Treatment for Chronic Kidney Disease
Yuan Min Wang and Stephen I. Alexander