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Nonribosomal peptide and polyketide biosynthesis

methods and protocols
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Year: 2016
Publisher: New York, NY, Humana Press
Series: Methods in molecular biology; 1401 , Springer protocols
Media group: Ausleihbestand
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Content

This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function. It then continues with methods for the analysis of these pathways including conventional enzymological assays, contemporary mass spectrometric analysis techniques, specialized molecular biological approaches applicable to NRPSs and PKSs, and small molecule analysis tools tailored to this very special class of natural products, and concludes by examining bioinformatics tools for the analysis of these enzymes, pathways, and molecules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nonribosomal Peptide and Polyketide Biosynthesis: Methods and Protocols serves as a valuable reference for those experienced in studying NRPS and PKS enzymes, pathways, and natural products as well as a gateway for those just entering the field.
 

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Statement of Responsibility: Editor Bradley S. Evans
Year: 2016
Publisher: New York, NY, Humana Press
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Classification: Search for this systematic NA-30, BC-40
Subject type: Search for this subject type Laborhandbuch
ISBN: 978-1-493-93373-0
ISBN (2nd): 1-493-93373-6
Description: xi, 281 Seiten : Illustrationen
Series: Methods in molecular biology; 1401 , Springer protocols
Tags: Proteine; Peptide; Enzymologie
Participating parties: Search for this character Evans, Bradley S. [Hrsg.]
Language: Englisch
Footnote: Literaturangaben
Media group: Ausleihbestand