Table of Contents
SECTION I. LABORATORY PROCEDURES FOR DETECTING VIRUSES
1. Quality Assurance in Clinical Virology
Christine C. Ginocchio
2. Specimen Requirements: Selection, Collection, Transport, and Processing
Thomas E. Grys and Thomas F. Smith
3. Primary Isolation of Viruses
Marie L. Landry
4. The Cytopathology of Virus Infection
Roger D. Smith and Anthony Kubat
5. Electron Microscopy and Immunoelectron Microscopy
Raymond Tellier, John Nishikawa, and Martin Petric
6. Immunofluorescence
Ted E. Schutzbank, Robyn McGuire, and David R. Scholl
7. Enzyme Immunoassays and Immunochromatography
Diane S. Leland
8. Immunoenzymatic Techniques for Detection of Viral Antigens in Cells and Tissue
Christopher R. Polage and Cathy A. Petti
9. Neutralization
David Schnurr
10. Hemadsorption and Hemagglutination-Inhibition
Stephen A. Young
11. Immunoglobulin M Determinations
Dean D. Erdman and Lia M. Haynes
12. Susceptibility Test Methods: Viruses
Max Q. Arens and Ella M. Swierkosz
13. Application of Western Blotting to Diagnosis of Viral Infections
Mark B. Meads and Peter G. Medveczky
14. Nucleic Acid Amplification and Detection Methods
Danny L. Wiedbrauk
15. Quantitative Molecular Techniques
Frederick S. Nolte
16. Flow Cytometry
James J. McSharry
SECTION II. VIRAL PATHOGENS
17. Respiratory Viruses
Christine Robinson
18. Enteroviruses and Parechoviruses
Mark A. Pallansch and M. Steven Oberste
19. Rotavirus, Caliciviruses, Astroviruses, Enteric Adenoviruses, and Other Viruses Causing Acute Gastroenteritis
Tibor Farkas and Xi Jiang
20. Waterborne Hepatitis
David A. Anderson
21. Blood-Borne Hepatitis Viruses: Hepatitis Viruses B, C, and D and Candidate Agents of Cryptogenetic Hepatitis
Mauro Bendinelli, Mauro Pistello, Fabrizio Maggi, and Marialinda Vatteroni
22. Rabies
Charles V. Trimarchi and Robert J. Rudd
23. Arboviruses
John T. Roehrig and Robert S. Lanciotti
24. Human Papillomaviruses
Raphael P. Viscidi and Keerti V. Shah
25. Human Polyomaviruses
Raphael P. Viscidi and Keerti V. Shah
26. Herpes Simplex Viruses
Laure Aurelian
27. Cytomegalovirus, Varicella-Zoster Virus, and Epstein-Barr Virus
Sonali K. Sanghavi, David T. Rowe, and Charles R. Rinaldo, Jr.
28. Human Herpesviruses 6, 7, and 8
Philip E. Pellett and Sheila C. Dollard
29. Poxviruses
Victoria A. Olson, Russell L. Regnery, and Inger K. Damon
30. Parvoviruses
Stanley J. Naides
31. Measles, Mumps, and Rubella
William J. Bellini and Joseph P. Icenogle
32. Human Retroviruses, Human Immunodeficiency Virus, and Human T-Lymphotropic Retrovirus (HTLV)
Jörg Schüpbach
33. Chlamydiae
Charlotte A. Gaydos
34. Rodent-Borne Viruses
Brian Hjelle and Fernando Torres-Perez
APPENDICES: REFERENCE LABORATORIES
A1. Virology Services Offered by the Federal Reference Laboratories at the Centers for Disease Control and Prevention
Brian W. J. Mahy
A2. State Public Health Laboratory Virology Services
Rosemary Humes