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Take your first steps in combinatorial synthesis or synthesize bioactive molecules such as antibiotics. Search for mutations in DNA using chemical probes or perform a proteome analysis in yeast.

The present selection of 12 inspiring experiments is tailored for maximum learning effect at minimal expense of time and equipment. Almost all currently used laboratory techniques in synthesis and analysis of bioactive compounds are represented at least once.

Abundant practical hints are complemented by a thorough treatment of the underlying theory and mechanisms.

Table of Contents

Introduction

DNA-Synthesis and DNA-Hybridization

Doubly Labeled Peptide Nucleic Acids as Probes for the Detection of DNA Point Mutations

Synthesis and Characterization of a Covalent Oligonucleotide-Streptavidin Conjugate for DNA-directed Immobilization

Solid Phase Synthesis of Peptides: Bradykinin Analogs and the Evaluation of Calcium Mobilization in PC-12 Cells

In silico Protein Ligand Design

Lipidation of Proteins and Peptides -

Farnesylation of the Ras protein

Insertion of Lipidated Peptides in Model Membranes

Isolation of Potato Phosphorylase and Enzymatic Synthesis of Amylose

Proteome Analysis -

Identification of Proteins Isolated from Yeast

Lectins -

Determination of the Sugar Specificity of Jacalin by a Sugar-Lectin Binding Assay

Combinatorial Chemistry -

Ugi-Synthesis, Genetic Algorithm, and Determination of Enzyme Inhibition

Solid-phase Synthesis of an Antibiotic

Glossary of Terms