Table of contents
Animal Cell Biotechnology - Methods and Protocols
Editor: Ralf Pörtner
Contents
Preface
Contributors
Part I. Basic techniques for establishing production cell lines
1 Transduction Technologies
David A. Fluri and Martin Fussenegger
2 Cells for gene therapy and vector production
C. Delenda, M. Chillon, A.-M. Douar and Otto-Wilhelm Merten
3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
Alexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier
4 DNA Fingerprinting and Characterisation of Animal Cell Lines
Glyn N. Stacey, Ed Byrne and J. Ross Hawkins
Part II. Basic cultivation techniques
5 Microcarrier Cell culture Technology
Gerald Blüml
6 Cell Encapsulation
Holger Hübner
7 Tools for High-Throughput Medium and Process Optimisation
Martin Jordan and Nigel Jenkins
Part III. Cell characterisation and analysis
8 Cell counting and viability measurements
Michael Butler and Maureen Spearman
9 Monitoring of Growth, Physiology and Productivity of animal cells by Flow Cytometry
Silvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai
10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
André A. Neves and Kevin M. Brindle
11 Methods for off-line analysis of nutrients and products in mammalian cell culture
Heino Büntemeyer
12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation
Derek Adams, Rashmi Korke, and Wei-Shou Hu
13 Measurement of apoptosis in cell culture
Adiba Ishaque1 and Mohamed Al-Rubeai
14 Metabolic Flux Estimation in Mammalian Cell Cultures
Chetan T. Goudar, Richard Biener, James M. Piret and Konstantin B. Konstantinov
Part IV. Special cultivation techniques
15 Disposable bioreactors for inoculum production and protein expression
Regine Eibl and Dieter Eibl
16 Hollow Fibre Cell Culture
John M. Davis
17 Cultivation of mammalian cells in fixed bed reactors
Ralf Pörtner and Oscar B.J. Platas Barradas
18 Configuration of Bioreactors
Dirk E. Martens and Evert Jan van den End
Part V. Downstream techniques
19 Membrane filtration in animal cell culture
Peter Czermak, Dirk Nehring and Ranil Wickramasinghe
20 Chromatographic techniques in the downstream processing of (recombinant) proteins
Ruth Freitag
Part VI. Special applications
21 Vaccine production - state of the art and future needs in upstream processing
Yvonne Genzel and Udo Reichl
22 Retrovirus production and characterization
Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues, Paula Alves
23 Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production
Sabine Geisse
Index
About this book
The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production.
The volumes five parts reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.
Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.
This book provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.