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Herpes simplex virus

methods and protocols
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Year: 2014
Publisher: New York, NY, Humana Press
Series: Methods in molecular biology; 1144 , Springer protocols
Media group: Ausleihbestand
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Content

Contents:
 
1. HSV-1 Biology and Life Cycle
 
Roger D. Everett
 
2. Herpes Simplex Virus Growth, Preparation, and Assay
 
Peggy Marconi and Roberto Manservigi
 
3. Isolation of Herpes Simplex Virus Nucleocapsid DNA
 
Moriah Szpara
 
4. Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes
 
Claus-Henning Nagel, Anja Pohlmann, and Beate Sodeik
 
5. Engineering HSV-1 Vectors for Gene Therapy
 
William F. Goins, Shaohua Huang, Justus B. Cohen, and Joseph C. Glorioso
 
6. Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors
 
Matias E. Melendez, Cornel Fraefel, and Alberto L. Epstein
 
7. HSV-1 Amplicon Vectors as Genetic Vaccines
 
Andrea S. Laimbacher and Cornel Fraefel
 
8. Modification of HSV-1 to an Oncolytic Virus
 
Hiroshi Nakashima and E. Antonio Chiocca
 
9. Herpes Simplex Virus Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo
 
Nancy M. Sawtell and Richard L. Thompson
 
10. Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals
 
Andreas Sauerbrei and Kathrin Bohn
 
11. Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation
 
Ju Youn Kim, Lora A. Shiflett, Jessica A. Linderman, Ian Mohr, and Angus C. Wilson
 
12. Characterization of Extracellular HSV-1 Virions by Proteomics
 
Roger Lippé
 
13. A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors
 
Kerstin Radtke, Fenja Anderson, and Beate Sodeik
 
14. Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein-Protein Interactions
 
David G. Meckes, Jr.
 
15. Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy
 
Monica Miranda-Saksena, Ross Boadle, and Anthony L. Cunningham
 
16. Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication
 
Michael Seyffert, Anna Paula de Oliveira, Cornel Fraefel, and Rebecca Vogel
 
17. Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination
 
Samuel D. Stampfer and Ekaterina E. Heldwein
 
18. Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components
 
Ryan H. Rochat, Corey W. Hecksel and Wah Chiu
 
19. In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells
 
Jolene M. Draper, Graham S. Stephenson and Jennifer H. LaVail
 
20. HSV-1 Protein Expression Using Recombinant Baculoviruses
 
Lorry M. Grady, Ping Bai and Sandra K. Weller
 
21. The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines
 
Joshua O. Marshak, Lichun Dong, and David M. Koelle
 

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Details

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Statement of Responsibility: edited ba Russell J. Diefenbach ; Cornel Fraefel
Year: 2014
Publisher: New York, NY, Humana Press
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ISBN: 978-1-493-90427-3
Description: XI, 331 S. : Ill., graph. Darst.
Series: Methods in molecular biology; 1144 , Springer protocols
Participating parties: Search for this character Diefenbach, Russell J. [Hrsg.]; Fraefel, Cornel [Hrsg.]
Language: Englisch
Footnote: Literaturangaben
Media group: Ausleihbestand