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Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents.Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, and 249 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design. Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition.

 

Audience

Students and professionals in biochemistry, biophysics, molecular biology, pharmacology, and pharmaceutical chemistry.

 

Contents

C.A. Fierke and G.G. Hammes, Transient Kinetic Approaches to Enzyme Mechanisms.R.D. Allison and D.L. Purich, Practical Considerations in the Design of Initial Velocity Enzyme Assays.C.Y. Huang, Derivation ofInitial Velocity and Isotope Exchange Rate Equations.F.B. Rudolph and H.J. Fromm, Plotting Methods for Analyzing Enzyme Rate Data.B. Mannervik, Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination between Rival Kinetic Models.K.E. Neet, Cooperactivity in Enzyme Function: Equilibrium and Kinetic Aspects.B.F. Cooper and F.B. Rudolph, Product Inhibition Applications.H.J. Fromm, Reversible Enzyme Inhibitors as Mechanistic Probes.A. Radzicka and R. Wolfenden, Transition State and Multisubstrate Analog Inhibitors.B.V. Plapp, Application of Affinity Labeling for Studying Structure and Function of Enzymes.R.B. Silverman, Mechanism-Based Enzyme Inactivators.D.L. Purich and R.D. Allison, Isotope Exchange Methods for Elucidating Enzymic Catalysis.L.S. Mullins and F.M. Raushel, Positional Isotope Exchange as a Probe of Enzyme Action. W.W. Cleland, Isotope Effects: Determination of Enzyme Transition State Structure.B.J. Bahnson and J.P. Klinman, Hydrogen Tunneling in Enzyme Catalysis.B.V. Plapp, Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis.D.L. Purich, Selected Exercises and Problems in Modern Enzyme Dynamics.