The synthesis of proteins by ribosomes is a fundamental cellular process. Cells must tightly control protein synthesis to maintain homeostasis and regulate proliferation, growth, differentiation, and development. Indeed, aberrant translational control is associated with cancer, several neurologic syndromes, and a group of genetic disorders termed “ribosomopathies.”
Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology covers our current understanding of all aspects of protein synthesis and its control. The contributors describe the fundamental steps in protein synthesis (initiation, elongation, and termination), the factors involved, and high-resolution structures of translational machinery where this takes place. They review the targets of translational control (e.g., initiation factors, mRNAs, and ribosomes) and how signaling pathways modulate this machinery. The roles of the endoplasmic reticulum, the unfolded protein response, processing bodies (P-bodies), stress granules, and small RNAs are also covered.
This volume includes discussion of translational deregulation in cancer and the development of therapeutic agents that target translation initiation. Thus, it is an essential reference for cell and molecular biologists, as well as cancer biologists and all those investigating human diseases associated with translation dysfunction.
Contents:
Preface
Principles of Translational Control: An Overview
John W.B. Hershey, Nahum Sonenberg, and Michael B. Mathews
The Structure and Function of the Eukaryotic Ribosome
Daniel N. Wilson and Jamie H. Doudna Cate
The Mechanism of Eukaryotic Translation Initiation: New Insights and Challenges
Alan G. Hinnebusch and Jon R. Lorsch
The Elongation, Termination, and Recycling Phases of Translation in Eukaryotes
Thomas E. Dever and Rachel Green
Single-Molecule Analysis of Translational Dynamics
Alexey Petrov, Jin Chen, Seán O’Leary, Albert Tsai, and Joseph D. Puglisi
The Current Status of Vertebrate Cellular mRNA IRESs
Richard J. Jackson
From Cis-Regulatory Elements to Complex RNPs and Back
Fátima Gebauer, Thomas Preiss, and Matthias W. Hentze
Regulation of mRNATranslation by Signaling Pathways
Philippe P. Roux and Ivan Topisirovic
Protein Secretion and the Endoplasmic Reticulum
Adam M. Benham
New Insights into Translational Regulation in the Endoplasmic Reticulum Unfolded Protein Response
Graham D. Pavitt and David Ron
P-Bodies and Stress Granules: Possible Roles in the Control of Translation and mRNA Degradation
Carolyn J. Decker and Roy Parker
mRNA Localization and Translational Control in Drosophila Oogenesis
Paul Lasko
Toward a Genome-Wide Landscape of Translational Control
Ola Larsson, Bin Tian, and Nahum Sonenberg
Imaging Translation in Single Cells Using Fluorescent Microscopy
Jeffrey A. Chao, Young J. Yoon, and Robert H. Singer
A Molecular Link between miRISCs and Deadenylases Provides New Insight into the Mechanism of Gene Silencing by MicroRNAs
Joerg E. Braun, Eric Huntzinger, and Elisa Izaurralde
Translational Control in Cancer Etiology
Davide Ruggero
Cytoplasmic RNA-Binding Proteins and the Control of Complex Brain Function
Jennifer C. Darnell and Joel D. Richter
Tinkering with Translation: Protein Synthesis in Virus-Infected Cells
Derek Walsh, Michael B. Mathews, and Ian Mohr
Emerging Therapeutics Targeting mRNATranslation
Abba Malina, John R. Mills, and Jerry Pelletier
Index