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Kurzbeschreibung

Written by one of the main contributors to progress in protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. It covers the theory, practice and latest achievements in x-ray crystallography, such that any researcher in structural biology will benefit from the contents.

 

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PART A THEORETICAL BASIS AND EXPERIMENTAL TECHNIQUES

Introduction

Techniques

Principles of X-ray Diffraction by a Crystal

Methods for Solving the Phase Problem

Model Building and Refinement

Time-resolved X-ray Crystallography

 

PART B CASE STUDIES FOR DIFFERENT KINDS OF X-RAY CRYSTAL STRUCTURE DETERMINATION OF BIOMACROMOLECULES

Space Group Determination and Data Reduction

Phase Determination by MIRAS Technique

Phase Determination by MAD Technique

Phase Determination by Molecular Replacement

Difference Fourier Technique

Map Improvement by NCS Averaging and other Methods

Automated Model Building

Structure Validation and PDB-Submission