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Multidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts.

 

Aus dem Inhalt

Preface.

 

1. Introduction, (Mark R. Schure and Steven A. Cohen).

 

Theory.

 

2. Elements of the Theory of Multidimensional Liquid Chromatography, (Mark R. Schure).

 

3. Peak capacity in Two-dimensional Liquid Chromatography (Joe M. Davis).

 

4. Decoding Complex 2-D Separations (Francseso Dondi, Maria Chiara Pietrogrande, Nicola Marchetti and Attila Felinger).

 

Columns, Instrumentation, and Methods.

 

5. Instrumentation for Comprehensive Multidimensional Liquid Chromatography (Robert. E. Murphy and Mark R. Schure).

 

6. Methods Development for Comprehensive Multidimensional Liquid Chromatography (Robert E. Murphy and Mark R. Schure).

 

7. Monolithic Columns and their 2D-HPLC applications (Tohru Ikegami, Hiroshi Aoki, Hiroshi Kimura, Ken Hosoya and Nobuo Tanaka).

 

8. Ultra-High Pressure Multidimensional Liquid Chromatography (Charles R. Evans and James W. Jorgenson).

 

Life Sciences Applications.

 

9. Peptidomics (Egidijus Machtejevas, Klaus K. Unger).

 

10. A Two-dimensional Liquid Mass Mapping Technique for Biomarker (David Lubman, Nathan S. Buchanan, Fred R. Miller, Kathleen Cho, Rong Wu, Steven Goodison, Yanfei Wang, Paweena Kreunin and Timothy J. Barder).

 

11. Coupled Multidimensional Chromatography and Tandem Mass Spectrometry Systems for Complex Peptide Mixture Analysis (Mike Washburn).

 

12. Development of Orthogonal 2D-LC Methods for Separations of Peptides (Martin Gilar, Petra Olivova, Amy E. Daly and John C. Gebler).

 

13. Protein IEX-RP-TOF MS (Steven A. Cohen and Scott Berger).

 

14. Analysis of Enantiomeric Compounds Using Multidimensional Liquid Chromatography (Renee J. Soukup and Dan Armstrong).

 

Multidimensional separations using CE.

 

15. Two-dimensional capillary electrophoresis for the comprehensive analysis of complex protein mixtures (James R. Kraly, Melissa M. Harwood, Megan Jones and Norman J. Dovici).

 

16. Two-dimensional HPLC-CE methods for protein/Peptide separation (Haleem Issaq).

 

Industrial Applications.

 

17. Multidimensional Liquid Chromatography in Industrial Applications (Frank Rittig and Harald Pasch).

 

18. The Analysis of Surfactants by Multidimensional Liquid Chromatography (Robert E. Murphy and Mark R. Schure.