Lars Christian Haeusler, Lars Hemsath, Dennis Fiegen, Lars Blumenstein, Ulrike Herbrand, Patricia Stege, Radovan Dvorsky and Mohammad Reza Ahmadian, Purification and Biochemical Properties of Rac1, 2, 3 and the Splice Variant Rac1b, Pages 1-11.
Adam Shutes, Anastacia C. Berzat, Emily J. Chenette, Adrienne D. Cox and Channing J. Der, Biochemical Analyses of the Wrch Atypical Rho Family GTPases, Pages 11-26.
Kirsti Hill and Heidi C.E. Welch, Purification of P-Rex1 from Neutrophils and Nucleotide Exchange Assay, Pages 26-41.
Jean-Francois Cote and Kristiina Vuori, In Vitro Guanine Nucleotide Exchange Activity of DHR-2/DOCKER/CZH2 Domains, Pages 41-57.
Daniel Baird, Qiyu Feng and Richard A. Cerione, Biochemical Characterization of the Cool (Cloned-Out-of-Library)/Pix (Pak-Interactive Exchange Factor) Proteins, Pages 58-69.
Bruno Mesmin and Bruno Antonny, GEF and Glucosylation Assays on Liposome-Bound Rac, Pages 70-80.
Celine M. DerMardirossian and Gary M. Bokoch, Phosphorylation of RhoGDI by p21-Activated Kinase 1, Pages 80-90.
Sonja Krugmann, Len Stephens and Phillip T. Hawkins, Purification of ARAP3 and Characterization of GAP Activities, Pages 91-103.
Erzsebet Ligeti and Jeffrey Settleman, Regulation of RhoGAP Specificity by Phospholipids and Prenylation, Pages 104-117.
Gerd Haug, Holger Barth and Klaus Aktories, Purification and Activity of the Rho ADP-Ribosylating Binary C2/C3 Toxin, Pages 117-127.
Erik Sahai and Michael F. Olson, Purification of TAT-C3 Exoenzyme, Pages 128-140.
Louis Hodgson, Perihan Nalbant, Feimo Shen and Klaus Hahn, Imaging and Photobleach Correction of Mero-CBD, Sensor of Endogenous Cdc42 Activation, Pages 140-156.
Andres M. Lebensohn, Le Ma, Hsin-Yi Henry Ho and Marc W. Kirschner, Cdc42 and PI(4,5)P2-Induced Actin Assembly in Xenopus Egg Extracts, Pages 156-173.
Hsin-Yi Henry Ho, Rajat Rohatgi, Andres M. Lebensohn and Marc W. Kirschner, In Vitro Reconstitution of Cdc42-Mediated Actin Assembly Using Purified Components, Pages 174-190.
Elizabeth S. Harris and Henry N. Higgs, Biochemical Analysis of Mammalian Formin Effects on Actin Dynamics, Pages 190-214.
James B. Moseley, Sankar Maiti and Bruce L. Goode, Formin Proteins: Purification and Measurement of Effects on Actin Assembly, Pages 215-234.
Hideyuki Mukai and Yoshitaka Ono, Purification and Kinase Assay of PKN, Pages 234-250.
Noriko Yokoyama and W. Todd Miller, Purification and Enzyme Activity of ACK1, Pages 250-260.
Jason P. Seifert, Jason T. Snyder, John Sondek and T. Kendall Harden, Direct Activation of Purified Phospholipase C Epsilon by RhoA Studied in Reconstituted Phospholipid Vesicles, Pages 260-271.
Jason T. Snyder, Mark R. Jezyk, Svetlana Gershburg, T. Kendall Harden and John Sondek, Regulation of PLC[beta] Isoforms by Rac, Pages 272-280.
Daisy W. Leung, David M. Morgan and Michael K. Rosen, Biochemical Properties and Inhibitors of (N-)WASP, Pages 281-296.
David Michaelson and Mark Philips, The Use of GFP to Localize Rho GTPases in Living Cells, Pages 296-315.
Takeshi Nakamura, Kazuo Kurokawa, Etsuko Kiyokawa and Michiyuki Matsuda, Analysis of the Spatiotemporal Activation of Rho GTPases Using Raichu Probes, Pages 315-332.
Fabian Oceguera-Yanez and Shuh Narumiya, Measurement of Activity of Rho GTPases During Mitosis, Pages 332-345.
Yukinori Endo, Sharona Even-Ram, Roumen Pankov, Kazue Matsumoto and Kenneth M. Yamada, Inhibition of Rho GTPases by RNA Interference, Pages 345-361.
Xinyu Chen and Ian G. Macara, RNA Interference Techniques to Study Epithelial Cell Adhesion and Polarity, Pages 362-374.
Annette Gaertner, Ludovic Collin and Giovanna Lalli, Nucleofection of Primary Neurons, Pages 374-388.
Mingjian Lu and Kodi S. Ravichandran, Dock180-ELMO Cooperation in Rac Activation, Pages 388-402.
Jennifer C. Erasmus and Vania M.M. Braga, Rho GTPase Activation by Cell-Cell Adhesion, Pages 402-415.
Hisakazu Ogita and Yoshimi Takai, Activation of Rap1, Cdc42, and Rac by Nectin Adhesion System, Pages 415-424.
Rafael Garcia-Mata, Krister Wennerberg, William T. Arthur, Nicole K. Noren, Shawn M. Ellerbroek and Keith Burridge, Analysis of Activated GAPs and GEFs in Cell Lysates, Pages 425-437.
Hong-Rui Wang, Abiodun A. Ogunjimi, Yue Zhang, Barish Ozdamar, Rohit Bose and Jeffrey L. Wrana, Degradation of RhoA by Smurf1 Ubiquitin Ligase, Pages 437-447.
Anne Doye, Laurent Boyer, Amel Mettouchi and Emmanuel Lemichez, Ubiquitin-Mediated Proteasomal Degradation of Rho Proteins by the CNF1 Toxin, Pages 447-456.
Ryu Takeya, Noriko Ueno and Hideki Sumimoto, Regulation of Superoxide-Producing NADPH Oxidases in Nonphagocytic Cells, Pages 456-468.
Zhui Chen and Melanie H. Cobb, Activation of MEKK1 by Rho GTPases, Pages 468-478.
Zhiheng Xu and Lloyd A. Greene, Activation of the Apoptotic JNK Pathway Through the Rac1-Binding Scaffold Protein POSH, Pages 479-489.
David M. Bourdon, Michele R. Wing, Earl B. Edwards, John Sondek and T. Kendall Harden, Quantification of Isozyme-Specific Activation of Phospholipase C-[beta]2 by Rac GTPases and Phospholipase C-[var epsilon] by Rho GTPases in an Intact Cell Assay System, Pages 489-499.
Raymond Habas and Xi He, Activation of Rho and Rac by Wnt/Frizzled Signaling, Pages 500-511.
Dana Lasko and Lisa McKerracher, Fluorescent Assay of Cell-Permeable C3 Transferase Activity, Pages 512-520.
Jean-Baptiste Manneville, Use of TIRF Microscopy to Visualize Actin and Microtubules in Migrating Cells, Pages 520-532.
Kirsi Riento and Anne J. Ridley, Inhibition of ROCK by RhoE, Pages 533-541.
Daniel R. Croft and Michael F. Olson, Conditional Regulation of a ROCK-Estrogen Receptor Fusion Protein, Pages 541-553.
Huzoor Akbar, Jose Cancelas, David A. Williams, Jie Zheng and Yi Zheng, Rational Design and Applications of a Rac GTPase-Specific Small Molecule Inhibitor, Pages 554-565.
Sandrine Etienne-Manneville, In Vitro Assay of Primary Astrocyte Migration as a Tool to Study Rho GTPase Function in Cell Polarization, Pages 565-578.
Edgar R. Gomes and Gregg G. Gundersen, Real-Time Centrosome Reorientation During Fibroblast Migration, Pages 579-592.
Justyna Janas, Jacek Skowronski and Linda Van Aelst, Lentiviral Delivery of RNAi in Hippocampal Neurons, Pages 593-605.
Xuemei Dong and Dianqing Wu, Methods for Studying Neutrophil Chemotaxis, Pages 605-613.
Qiong Lin, Wannian Yang and Richard A. Cerione, Measurement of Epidermal Growth Factor Receptor Turnover and Effects of Cdc42, Pages 614-625.
Steven Hooper, John F. Marshall and Erik Sahai, Tumor Cell Migration in Three Dimensions, Pages 625-643.
Jaime Millan, Lynn Williams and Anne J. Ridley, An In Vitro Model to Study the Role of Endothelial Rho GTPases During Leukocyte Transendothelial Migration, Pages 643-655.
Shingo Yasuda and Shuh Narumiya, Analysis of a Mitotic Role of Cdc42, Pages 656-665.
Laura J. Turner and Alan Hall, Plexin-Induced Collapse Assay in COS Cells, Pages 665-676.
Lucy Erin O'Brien, Wei Yu, Kitty Tang, Tzuu-Shuh Jou, Mirjam M.P. Zegers and Keith E. Mostov, Morphological and Biochemical Analysis of Rac1 in Three-Dimensional Epithelial Cell Cultures, Pages 676-691.
Bin Xiang and Senthil K. Muthuswamy, Using Three-Dimensional Acinar Structures for Molecular and Cell Biological Assays, Pages 692-701.
Shian-Huey Chiang, Louise Chang and Alan R. Saltiel, TC10 and Insulin-Stimulated Glucose Transport, Pages 701-714.
Jens C. Schwamborn, Yinghua Li and Andreas W. Pïœschel, GTPases and the Control of Neuronal Polarity, Pages 715-727.
Danijela Vignjevic, John Peloquin and Gary G. Borisy, In Vitro Assembly of Filopodia-Like Bundles, Pages 727-739.