Contents
Preface v
Contributors ix
1 Blood/Brain Barrier and Cell/Cell Interactions: Methods
for Establishing In Vitro Models of the Blood/Brain Barrier
and Transport Measurements
Michael Aschner, Vanessa A. Fitsanakis, Ana Paula Marreilha dos Santos, Luisa Olivi,
and Joseph P. Bressler 1
2 Interactions of Intestinal Epithelial Cells With Bacteria
and Immune Cells: Methods to Characterize Microflora
and Functional Consequences
Geraldine Canny, Alexander Swidsinski, and Beth A. McCormick x
3 The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
Triantafyllos Chavakis and Valeria Orlova xx
4 Cell/Cell Interactions on Solid Matrices
Nancy A. Louis, Dionne Daniels, and Sean P. Colgan xx
5 Cell/Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein _-Subunits
in MDCK Cells for Studies of Epithelial Cell Junction Structure and Function
Ernesto Sabath and Bradley M. Denker xx
6 Nucleotide Metabolism and Cell/Cell Interactions
Holger K. Eltzschig, Thomas Weissmuller, Alice Mager,
and Tobias Eckle xx
7 Analysis of Mammallian Sperm/Egg Membrane Interactions
During In Vitro Fertilization
Genevieve B. Wortzman, Allison J. Gardner, and Janice P. Evans xx
8 Collagen Gel Contraction Assay
Peter Ngo, Punitha Ramalingham, Jonathan Phillips,
and Glenn T. Furuta xxx
9 Methods to Assess Tissue Permeability
Juan C. Ibla and Joseph K. Khoury xxx
11 A Biochemical Method for Tracking Cholera Toxin Transport from Plasma Membrane to
Golgi and Endoplasmic Reticulum
Heidi E. DeLuca and Wayne I. Lencer
12 Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model
Systems
Yaw-Chyn Lim and Francis W. Luscinskas
13 Expression Cloning of Signaling Proteins Regulated by Cell Adhesion
Michelle L. Matter, and Joe W. Ramos
14 Assays for the Calcium Sensitivity of Desmosomes
Anita J. Merritt, Anthea Scothern, and Tanusree Bhattacharyya
15 Tight Junctions and Cell{-}Cell Interactions
Markus Utech, Matthias Bruwer, and Asma Nusrat
16 Simultaneous Immunofluorescence Detection of Cadherin and _-catenin in Mouse Tissue
Sections
Nobuko Obara
17 In Vitro Neutrophil Transepithelial Migration
Winston Y. Lee, Alex C. Chin, Susan Voss, and Charles A. Parkos
18 The Blot Running Assay: A Method for Identifying Adhesion Molecules Mediating Binding
Under Shear Conditions
Robert Sackstein and Robert Fuhlbrigge
19 Cell{-}Cell Interaction in the Transcellular Biosynthesis of Novel _-3-Derived Lipid
Mediators
Nan Chiang and Charles N. Serhan
20 Isolation and Analysis of Lipid Rafts in Cell{-}Cell Interactions
Aimee Landry and Ramnik Xavier
Index 385