Presents a global overview of viral (DNA, retro and RNA) interactions with the nucleus and its components.
Includes contributions from the world's leading experts in their fields
The definitive text on viral interactions with the nucleus
Presents_a gallery of stunning microscopic images to illustrate the diversity of viral nuclear interactions in full colour.
Contents
Preface ix
Contributors xi
1 The Nucleus - An Overview 1
Dean A. Jackson
1.1 Introduction 1
1.2 The gene expression pathway in mammalian cells 2
1.3 Chromosome structure and DNA loops 9
1.4 Duplicating the genetic code 9
1.5 Nuclear compartmentalization 10
1.6 Nuclear architecture - implications for
viral infection 18
1.7 Conclusion 19
References 20
2 The Eukaryotic Cell Cycle 25
Jane V. Harper and Gavin Brooks
2.1 Introduction 25
2.2 The G0/G1 transition 31
2.3 The G1-S transition 32
2.4 S phase 39
2.5 The G2-M transition 45
2.6 Mitosis (M-phase) 48
2.7 Cytokinesis 52
2.8 Endoreduplication 53
2.9 Summary and conclusions 53
References 54
3 DNA Viruses and the Nucleus 69
G. Eric Blair and Nicola James
3.1 Introduction 69
3.2 The structure of the nucleus 70
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3.3 DNA viruses and the nucleus 75
3.4 Concluding comments and future directions 81
References 81
4 Retroviruses and the Nucleus 89
Carlos de Noronha and Warner C. Greene
4.1 Retroviral structure 91
4.2 The retroviral life cycle 91
4.3 Entering the nucleus 93
4.4 Inside the nucleus 101
4.5 Leaving the nucleus and the cell 113
4.6 Future perspectives 114
References 114
5 Negative-sense RNA Viruses and the Nucleus 129
Debra Elton and Paul Digard
5.1 Introduction 129
5.2 The necessity of a nucleus 132
5.3 Nuclear import machinery 133
5.4 The cellular transcription machinery 135
5.5 Nuclear architecture 140
5.6 Nuclear export 143
5.7 Evasion of innate antiviral responses 147
References 148
6 Positive-strand RNA Viruses and the Nucleus 161
Kurt E. Gustin and Peter Sarnow
6.1 Introduction 161
6.2 Replication of positive-strand RNA viruses
in enucleated cells 162
6.3 Localization of viral proteins to the nucleus 163
6.4 Interaction of nuclear factors with viral proteins
and nucleic acids 165
6.5 Cytoplasmic accumulation of nuclear proteins
during infection 168
6.6 Disruption of nucleo-cytoplasmic trafficking
by positive-strand RNA viruses 169
6.7 Advantages provided to the virus by inhibiting
nuclear import 172
6.8 Conclusions 175
References 178
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CONTENTS vii
7 Viruses and the Nucleolus 185
David A. Matthews and Julian A. Hiscox
7.1 Introduction 185
7.2 Techniques used to examine the nucleolus 191
7.3 Nucleolar localization signals (NoLS) 191
7.4 Viral interactions with the nucleolus 192
7.5 The nucleolus, viruses and autoimmunity 201
7.6 Summary 201
References 202
8 Virus Interactions with PML Nuclear Bodies 213
Keith N. Leppard and John Dimmock
8.1 Introduction 213
8.2 ND10 in uninfected cells 213
8.3 Functions of ND10 217
8.4 ND10 and virus infection 219
8.5 Why do viruses interact with and modify ND10? 232
References 235
9 Viruses and the Cell Cycle 247
Crisanto Gutierrez, Brian Dove and Julian A. Hiscox
9.1 Introduction: an overview of the eukaryotic cell cycle 247
9.3 Retroviruses and the cell cycle 254
9.4 RNA viruses and the cell cycle 255
9.5 Viruses, the nucleolus and cell cycle control 259
9.6 Viral interaction with activator protein-1 (AP-1) 259
9.7 Summary 260
References 262
Index 271