Table of contents
Table of Contents
Cancer Genomics and Proteomics: Methods and Protocols, Paul B. Fisher, Ed.
1. Devanand Sarkar, Dong-chul Kang and Paul B. Fisher
Reciprocal Subtraction Differential RNA Display (RSDD): An Efficient Technology for Cloning Differentially Expressed Genes
2. Habib Boukerche, Zao-zhong Su, Dong-chul Kang & Paul B. Fisher
Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH)
3. Ken C.N. Chang, Barry Komm, Nichole Boyer Arnold, and Murray Korc
The Application of Differential Display as a Gene Profiling Tool
4. Fred van Ruissen and Frank Baas
Serial Analysis of Gene Expression (SAGE)
5. Katia Basso, and Riccardo Dalla-Favera
Gene expression profile analysis of tumors
6. Zhong Chen, Tin-Lap Lee, Xin-Ping Yang, Gang Dong, Amy Loercher, and Carter Van Waes
cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-KappaB Related Genes in Squamous Cell Carcinoma
7. Yan Ma, Qing Feng, Ian Pitha-Rowe, Sutisak Kitareewan and
Ethan Dmitrovsky
Gene Profiling Uncovers Retinoid Target Genes
8. Dong-chul Kang and Paul B. Fisher
Complete Open Reading Frame (C-ORF) Technique: A Rapid and Efficient Method for Obtaining Complete Protein Coding Sequences
9. Piyali Dasgupta and Srikumar P. Chellappan
Chromatin Immunoprecipitation Assays: Molecular Analysis of Chromatin Modification and Gene Regulation
10. Stephanie F. Phelps, Sharon Illenye and Nicholas H. Heintz
Manipulating Genes and Gene Copy Number by BAC Transfection
11. Pulivarthi H. Rao, Subhadra V. Nandula, and Vundavalli V. Murty
Molecular Cytogenetic Applications in the Analysis of Cancer Genome
12. Hetty Carraway and James Herman
Evaluation of DNA Methylation
13. Renee Brissette and Neil I. Goldstein
The Use of Phage Display Peptide Libraries for Basic and Translational Research
14. Shu-ichi Matzsuzawa and John C. Reed
Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions
15. Timothy E. Baroni, Michele T. Lastro, Aparna C. Ranganathan, Scott A.
Tenenbaum, Douglas S. Conkin and Julio Aguirre-Ghiso
Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associated with Tumor Growth Arrest
16. Neil I. Goldstein and Paul B. Fisher
Surface-Epitope Masking (SEM): An Immunological Subtraction Approach for Developing Monoclonal Antibodies Targeting Surface-Expressed Molecules
17. Paul Dent, Philip B. Hylemon, Steven Grant and Paul B. Fisher
Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells
18. Siddharth Balachandran and Glen N. Barber
PKR in Innate Immunity, Cancer, and Viral Oncolysis
19. Hong Sun and Reshma Taneja
Analysis of Transformation and Tumorigenicity using Mouse Embryonic Fibroblast Cells
20. Hong Sun, Neriman Tuba Gulbagci and Reshma Taneja-
Analysis of Growth Properties and Cell Cycle Regulation using Mouse Embryonic Fibroblast Cells
21. Virginia Espina, Julia Wulfkuhle, Emanuel F. Petricoin III & Lance A. Liotta
Reverse Phase Protein Microarrays for Monitoring Biologic Responses
22. Champion Deivanayagam, William J. Cook and Mark R. Walter
Protein Crystallization
About this book
Cancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function. The approaches described in this volume are state-of-the-art and may be tailored to ongoing individual or planned research projects.
Reviews are written by experts in specific aspects of gene identification and full-length gene cloning, gene profiling (microarrays), chromatin modification of gene regulation, bacterial artificial chromosomes, cancer cytogenetic analyses, and gene methylation. Chapters also discuss topics such as phage display, yeast and mammalian two-hybrid systems, RNA silencing, monoclonal antibody production, kinases and signal transduction, PKR, analyses of mouse embryo fibroblasts, protein microarrays, and protein crystallization.
Cancer Genomics and Proteomics: Methods and Protocols will be of interest to molecular biologists, geneticists, cell biologists, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.