Zweigstelle	Standorte	Status	Vorbestellungen	Frist	Barcode	Lageplan	Ausleihhinweis
Zweigstelle: Dauerleihe	Standorte: GE-40 285	Status: Verfügbar	Vorbestellungen: 0	Frist:	Barcode: 00264202	Lagepläne: Lageplan	Ausleihhinweis:

The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent on having both editions in order to utilize the system. The chapters in this indispensable laboratory aid cover a wide range of topics associated with Pichia Pastoris. The use of fermentors to grow Pichia Pastoris and good advice on how to go about the initial processing of material from these cultures is discussed. An extensive discussion on how to formally analyze mutations in essential P. pastoris genes is also presented. The final chapters are for cell biologists who are for cell biologists using the Pichia Pastoris system to investigate protein interactions, or to localize proteins in yeast cells. Pichia Protocols 2nd Edition, is an essential tool for researchers working with the Pichia Pastoris system

TABLE OF CONTENTS

Table of Contents

 

Chapter 1 - Introduction [REVIEW CHAPTER]

 

Chapter 2 - Vectors and strains for expression [REVIEW CHAPTER]

 

Chapter 3 - DNA-Mediated Transformation

 

Chapter 4 - Rational design and optimization of fed-batch and continuous fermentations

 

Chapter 5 - Overexpression of a hookworm secretory protein saturates secretory capacity

 

Chapter 6 - Initial processing and purification strategies

 

Chapter 7 - Rapid screening of chromatography resins for the purification of proteins

 

Chapter 8 - Characterization of O-linked saccharides on glycoproteins

 

Chapter 9 - Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids

 

Chapter 10 - N-linked glycan characterization of heterologous proteins

 

Chapter 11 - Heavy labeling of recombinant proteins

 

Chapter 12 - Selenomethionine labeling of recombinant proteins

 

Chapter 13 - Deuterium labeling of proteins

 

Chapter 14 - Classical genetic analysis

 

Chapter 15 - Identification of pexophagy genes by Restriction Enzyme-Mediated Integration (REMI)

 

Chapter 16 - Characterization of protein-protein interactions

 

Chapter 17 - Localization of proteins and organelles using fluorescence microscopy

 

Chapter 18 - Fluorescence microscopy and thin-section electron microscopy