About this book
This book presents a compendium of methodologies for the study of membrane lipids, varying from traditional lab bench experimentation to computer simulation and theoretical models. This volume provides a comprehensive set of techniques for studying membrane lipids, and their interactions with other membrane lipids, with membrane proteins, and with exogenous agents such as anesthetics. The progression of chapters increases in complexity, initially presenting introductory concepts and methods for the study of simple structures and interactions, then moving to the study of more complex structures and interactions, including: behaviors of lipids in water systems and phases; membrane lipid movements and diffusion; properties of lipids in mono and bilayers; complex lipid domains; and membrane-lipid interaction with proteins and drugs. Each chapter is written by an internationally recognized expert in the field.
Written for:
Biochemists, molecular and cell biologists, cell biophysicists, pharmacologists, neurochemists, neuropharmacologists, anasthesiologists
Table of contents
Methods in Membrane Lipids: Table of Contents
Introductory Chapters:
1 A glance at the structural and functional diversity of membrane lipids
Alejandro M. Dopico and Gabor J. Tigyi
2 Membrane lipid polymorphism: relationship to bilayer properties and
protein function
Richard Epand
Lipid distribution, membrane lipid structure, and lipid-lipid
interactions:
3 Acrylodan-labeled intestinal fatty acid binding protein to measure
concentrations of unbound fatty acids
Jeffrey R. Simard, Frits Kamp, and James A. Hamilton
4 Measuring molecular order and orientation using Coherent-anti Stokes
Raman scattering
microscopy
Hilde A. Rinia, George W.H. Wurpel, and Michiel Müller
5 Preparation of oriented, fully hydrated lipid samples for structure
determination using X-ray scattering
Stephanie Tristram-Nagle
6 Nuclear Magnetic Resonance investigation of oriented lipid membranes
Olivier Soubias and Klaus Gawrisch
7 Molecular dynamics simulations as a complement to Nuclear Magnetic
Resonance and X-ray diffraction measurements
Scott Feller
8 Use of inverse theory algorithms in the analysis of biomembrane
Nuclear Magnetic Resonance data
Edward Sternin
9 Statistical thermodynamics via computer simulation to characterize
phospholipid interactions in membranes
Mihaly Mezei and Pál Jedlovszky
10 Fluorometric assay for detection of sterol oxidation in liposomal
membranes
Parkson L-G. Chong and Michelle Olsher
11 Fluorescence detection of signs of sterol superlattice formation in
lipid membranes
Parkson L-G. Chong, Berenice Venegas, and Michelle Olsher
Characterization of lipid phases:
12 Differential scanning calorimetry in the study of lipid phase
transitions in model and biological membranes: practical considerations
Ruthven N. A. H. Lewis, David A. Mannock, and Ronald N. McElhaney
13 Pressure-perturbation calorimetry
Heiko Heerklotz
14 Fourier transform infrared spectroscopy in the study of lipid phase
transitions in model and biological membranes: practical considerations
Ruthven N. A. H.Lewis and Ronald McElhaney
15 Optical dynamometry to study phase transitions in lipid membranes
Rumiana Dimova and Bernard Pouligny
Lipid movements and diffusion:
16 Fluorescence assays for measuring fatty acid binding and transport
through membranes
Kellen Brunaldi, Jeffrey R. Simard, Frits Kamp, Charu Rewal, Tanong
Asawakarn, Paul O'Shea, and James A. Hamilton
17 Measurement of lateral diffusion rates in membranes by Pulsed
Magnetic Field Gradient, Magic Angle-Spinning Proton Nuclear Magnetic
Resonance
Klaus Gawrisch and Holly Gaede
18 Using Fluorescence Recovery After Photobleaching to measure
diffusion in membranes
Conrad W. Mullineaux and Helmut Kirchhoff
19 Single molecule fluorescence microscopy to determine phospholipid
lateral diffusion
Michael J. Murcia, Sumit Garg, and Christoph Naumann
20 Modeling two- and three-dimensional diffusion
Michael Saxton
21 Measurement of water and solute permeability by stopped-flow
fluorimetry
John C. Mathai and Mark L. Zeidel
Pressure between lipids, mono- and bilayer lipid curvature and stress:
22 Fluorescence microscopy to study pressure between lipids in giant
unilamelar vesicles
Anna Celli, Claudia Y.C. Lee, and Enrico Gratton
23 X-ray scattering and solid-state deuterium Nuclear Magnetic
Resonance probes of structural fluctuations in lipid membranes
Horia I. Petrache and Michael F. Brown
24 Determination of lipid spontaneous curvature from X-ray examinations
of inverted hexagonal phases
Michael Kozlov
25 Shape analysis of giant vesicles with fluid phase coexistence by
laser scanning microscopy to determine curvature, bending elasticity
and line tension
Samuel T. Hess, Manasa V. Gudheti, Michael Mlodzianoski, and Tobias
Baumgart
26 Laser tweezer deformation of giant unilamellar vesicles
Cory Poole and Wolfang Losert
27 Measurement of lipid forces by X-ray diffraction and osmotic stress
Horia I. Petrache, Daniel Harries, and V. Adrian Parsegian
28 Micropipette aspiration for measuring elastic properties of lipid
bilayers
Marjorie L. Longo and Hung V. Ly
29 Langmuir films to determine lateral surface pressure on lipid
segregation
Antonio Cruz and Jesús Pérez-Gil
Lipid domains:
30 Detergent and detergent-free methods to define lipid rafts and
caveolae
Rennolds S. Ostrom and Xiaoqiu Liu
31 Near field scanning optical microscopy to identify membrane
microdomains
Anatoli Ianoul and Linda Johnston
32 Fluorescence microscopy to study domains in supported lipid bilayers
Jonathan M. Crane and Lukas K. Tamm
33 Fluorescence resonance energy transfer to characterize
cholesterol-induced domains
Luis M.S. Moura and Manuel Prieto
34 Lipid domains in supported lipid bilayers for atomic force microscopy
Wan-Chen Lin, Craig D. Blanchette, Timothy V. Ratto, and Marjorie L.
Longo
Membrane lipid-protein/drug interactions:
35 Nuclear Magnetic Resonance structural studies of membrane proteins
in micelles and bilayers
Xiao-Min Gong, Carla M. Franzin, Khang Thai, Jinghua Yu, and Francesca
Marassi
36 Laurdan studies of membrane lipid-acetylcholine receptor protein
interactions
Silvia S. Antollini and Francisco J. Barrantes
37 Single-molecule methods for monitoring changes in bilayer elastic
properties
Olaf S. Andersen, Michael J. Bruno, Roger E. Koeppe II, and Haiyan Sun
38 Ion channel reconstitution
Francisco J. Morera, Guillermo Vargas, Carlos González, Eduardo
Rosenmann, and Ramón Latorre
39 The use of differential scanning calorimetry to study drug-membrane
interactions
Thomas M. Mavromoustakos
40 Atomic force microscopy to study interacting forces in phospholipid
bilayers containing general anesthetics
Zoya V. Leonenko, Eric Finot, and David T. Cramb