Holger Erfle and Rainer Pepperkok, Arrays of Transfected Mammalian Cells for High Content Screening Microscopy, Pages 1-8.
Rainer Pepperkok, Jeremy C. Simpson, Jens Rietdorf, Cihan Cetin, Urban Liebel, Stefan Terjung and Timo Zimmermann, Imaging Platforms for Measurement of Membrane Trafficking, Pages 8-18.
Urban Liebel, Bjoern Kindler and Rainer Pepperkok, Bioinformatic "Harvester": A Search Engine for Genome-Wide Human, Mouse, and Rat Protein Resources, Pages 19-26.
Brian Storrie, Microinjection as a Tool to Explore Small GTPase Function, Pages 26-42.
Alexander A. Mironov, Galina V. Beznoussenko, Alberto Luini and Roman S. Polishchuk, Visualizing Intracellular Events In Vivo by Combined Video Fluorescence and 3-D Electron Microscopy, Pages 43-57.
Cemal Gurkan and William E. Balch, Recombinant Production in Baculovirus-Infected Insect Cells and Purification of the Mammalian Sec13/Sec31 Complex, Pages 58-66.
Paul LaPointe and William E. Balch, Purification and Properties of Mammalian Sec23/24 from Insect Cells, Pages 66-74.
Eugene Futai and Randy Schekman, Purification and Functional Properties of Yeast Sec12 GEF, Pages 74-82.
Ken Sato and Akihiko Nakano, Reconstitution of Cargo-Dependent COPII Coat Assembly on Proteoliposomes, Pages 83-94.
Joelle Bigay and Bruno Antonny, Real-Time Assays for the Assembly-Disassembly Cycle of COP Coats on Liposomes of Defined Size, Pages 95-107.
Meir Aridor, Kuntala Shome and Guillermo Romero, Assay and Measurement of Phospholipase D Activation by Sar1, Pages 108-115.
William J. Brown and John A. Schmidt, Use of Acyltransferase Inhibitors to Block Vesicular Traffic Between the ER and Golgi Complex, Pages 115-125.
Ayano Satoh, Jörg Malsam and Graham Warren, Tethering Assays for COPI Vesicles Mediated by Golgins, Pages 125-134.
Holly Hoover, Vandhana Muralidharan-Chari, Sarah Tague and Crislyn D'Souza-Schorey, Investigating the Role of ADP-Ribosylation Factor 6 in Tumor Cell Invasion and Extracellular Signal-Regulated Kinase Activation, Pages 134-147.
Magnus Mutah Che, Zhongzhen Nie and Paul A. Randazzo, Assays and Properties of the Arf GAPs AGAP1, ASAP1, and Arf GAP1, Pages 147-163.
Vi Luan Ha, Geraint M.H. Thomas, Stacey Stauffer and Paul A. Randazzo, Preparation of Myristoylated Arf1 and Arf6, Pages 164-174.
Heather D. Jones, Joel Moss and Martha Vaughan, BIG1 and BIG2, Brefeldin A-Inhibited Guanine Nucleotide-Exchange Factors for ADP-Ribosylation Factors, Pages 174-184.
Gustavo Pacheco-Rodriguez, Joel Moss and Martha Vaughan, Cytohesin-1: Structure, Function, and ARF Activation, Pages 184-195.
Alessandro Vichi, Joel Moss and Martha Vaughan, ADP-Ribosylation Factor Domain Protein 1 (ARD1), a Multifunctional Protein with Ubiquitin E3 Ligase, GAP, and ARF Domains, Pages 195-206.
Hye-Won Shin, Chisa Shinotsuka and Kazuhisa Nakayama, Expression of BIG2 and Analysis of Its Function in Mammalian Cells, Pages 206-215.
Shigeru Hashimoto, Ari Hashimoto, Atsuko Yamada, Yasuhito Onodera and Hisataka Sabe, Assays and Properties of the ArfGAPs, AMAP1 and AMAP2, in Arf6 Function, Pages 216-231.
Hiroyuki Sakagami, Akifumi Kamata, Kohji Fukunaga and Hisatake Kondo, Functional Assay of EFA6A, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 6 (ARF6), in Dendritic Formation of Hippocampal Neurons, Pages 232-242.
Delia J. Hernandez-Deviez and Jean M. Wilson, Functional Assay of ARNO and ARF6 in Neurite Elongation and Branching, Pages 242-252.
Kanamarlapudi Venkateswarlu, Analysis of the Interaction Between Cytohesin 2 and IPCEF1, Pages 252-266.
Ivan de Curtis and Simona Paris, Assay and Properties of the GIT1/p95-APP1 ARFGAP, Pages 267-278.
Ayano Satoh, Matthew Beard and Graham Warren, Preparation and Characterization of Recombinant Golgin Tethers, Pages 279-296.
Carmen Valente, Stefania Spane, Alberto Luini and Daniela Corda, Purification and Functional Properties of the Membrane Fissioning Protein CtBP3/BARS, Pages 296-316.
Hye-Young Yoon, Juan S. Bonifacino and Paul A. Randazzo, In Vitro Assays of Arf1 Interaction with GGA Proteins, Pages 316-332.
Frederic Luton, The Role of EFA6, Exchange Factor for Arf6, for Tight Junction Assembly, Functions, and Interaction with the Actin Cytoskeleton, Pages 332-345.
Ji-Long Chen, Weidong Xu and Mark Stamnes, In Vitro Reconstitution of ARF-Regulated Cytoskeletal Dynamics on Golgi Membranes, Pages 345-358.
Ok-Ho Shin and John H. Exton, Assays and Properties of Arfaptin 2 Binding to Rac1 and ADP-Ribosylation Factors (Arfs), Pages 359-367.
Kazuhisa Nakayama and Hiroyuki Takatsu, Analysis of Arf Interaction with GGAs In Vitro and In Vivo, Pages 367-377.
Alice Y. Welch, Kathleen N. Riley, Crislyn D'Souza-Schorey and Ira M. Herman, Arf6 Modulates the [beta]-Actin Specific Capping Protein, [beta]cap73, Pages 377-387.
Michael Krauss and Volker Haucke, Functional Assay of Effectors of ADP Ribosylation Factor 6 During Clathrin/AP-2 Coat Recruitment to Membranes, Pages 388-398.
Dale J. Powner, Trevor R. Pettitt and Michael J.O. Wakelam, Assays to Study Phospholipase D Regulation by Inositol Phospholipids and ADP-Ribosylation Factor 6, Pages 398-410.
Anamarija Kruljac-Letunic and Andree Blaukat, Assay and Functional Properties of the Tyrosine Kinase Pyk2 in Regulation of Arf1 Through ASAP1 Phosphorylation, Pages 411-422.
Yoshikatsu Aikawa and Thomas F.J. Martin, ADP-Ribosylation Factor 6 Regulation of Phosphatidylinositol-4,5-Bisphosphate Synthesis, Endocytosis, and Exocytosis, Pages 422-431.
Lei Lu, Guihua Tai and Wanjin Hong, Interaction of Arl1 GTPase with the GRIP Domain of Golgin-245 as Assessed by GST (Glutathione-S-transferase) Pull-Down Experiments, Pages 432-441.
Guihua Tai, Lei Lu, Ludger Johannes and Wanjin Hong, Functional Analysis of Arl1 and Golgin-97 in Endosome-to-TGN Transport Using Recombinant Shiga Toxin B Fragment, Pages 442-453.
J. Bradford Bowzard, J. Daniel Sharer and Richard A. Kahn, Assays Used in the Analysis of Arl2 and Its Binding Partners, Pages 453-467.
R. Jane Evans, J. Paul Chapple, Celene Grayson, Alison J. Hardcastle and Michael E. Cheetham, Assay and Functional Analysis of the ARL3 Effector RP2 Involved in X-Linked Retinitis Pigmentosa, Pages 468-480.
Rene Bartz, Corinne Benzing and Oliver Ullrich, Reconstitution of Transport to Recycling Endosomes In Vitro, Pages 480-490.
Marilyn Leonard, Byeong Doo Song, Rajesh Ramachandran and Sandra L. Schmid, Robust Colorimetric Assays for Dynamin's Basal and Stimulated GTPase Activities, Pages 490-503.
Ishido Miwako and Sandra L. Schmid, Clathrin-Coated Vesicle Formation from Isolated Plasma Membranes, Pages 503-511.
Simone Kunzelmann, Gerrit J.K. Praefcke and Christian Herrmann, Nucleotide Binding and Self-Stimulated GTPase Activity of Human Guanylate-Binding Protein 1 (hGBP1), Pages 512-527.
Yumi Yoshida and Kohji Takei, Stimulation of Dynamin GTPase Activity by Amphiphysin, Pages 528-537.
Gianluca Cestra, Adam Kwiatkowski, Marco Salazar, Frank Gertler and Pietro De Camilli, Tuba, A GEF for CDC42, Links Dynamin to Actin Regulatory Proteins, Pages 537-545.
Richard Lundmark and Sven R. Carlsson, Expression and Properties of Sorting Nexin 9 in Dynamin-Mediated Endocytosis, Pages 545-556.
Annie Quan and Phillip J. Robinson, Rapid Purification of Native Dynamin I and Colorimetric GTPase Assay, Pages 556-569.
Sanja Sever, Jesse Skoch, Brian J. Bacskai and Sherri L. Newmyer, Assays and Functional Properties of Auxilin-Dynamin Interactions, Pages 570-585.
Michael Schrader and Stephen J. Gould, Assay and Functional Analysis of Dynamin-Like Protein 1 in Peroxisome Division, Pages 586-597.
Matthew K. Higgins and Harvey T. McMahon, In Vitro Reconstitution of Discrete Stages of Dynamin-dependent Endocytosis, Pages 597-611.
Elena Ingerman and Jodi Nunnari, A Continuous, Regenerative Coupled GTPase Assay for Dynamin-Related Proteins, Pages 611-619.
Lorena Griparic and Alexander M. van der Bliek, Assay and Properties of the Mitochondrial Dynamin Related Protein Opa1, Pages 620-631.
Georg Kochs, Mike Reichelt, Dganit Danino, Jenny E. Hinshaw and Otto Haller, Assay and Functional Analysis of Dynamin-Like Mx Proteins, Pages 632-643.