Written by an international group of eminent scientists, this new treatise is the very first in the field to provide a thorough, state-of-the-art review of the periplasm, the extracytoplasmic compartment found in gram-negative bacteria. Targeting medical practitioners and students of biology, microbiology, bichemistry, structural biology and biotechnology, The Periplasm detials important aspects of the physiology of pathogenic microorganisms, conveys a selection of current drug resistance strategies, and describes lipopolysaccharide biosynthesis.
Valuable background information outlining the inner workings of gram-negative bacteria is presented, providing insights into the evolution of cellular compartments and their benefit to living organisms. There are also comprehensive discussions of the basic biological functions of the periplasm and their physiological relevance, including protein transport, folding, and quality control; bioenergetics; solute transport; stress responses; cell division; and cell architecture.
The Periplasm lays the foundation for future research directions and novel experiments in its coverage of the most up-to-date results, approaches, and hypotheses available to those working in the field. Recent insights into computational methods to predict cellular localization and the latest developments in recombinant protein production are also examined.
Key Features
Examines the mechanisms and physiological relevance of the basic biological functions of the periplasm
Provides insights into the evolution of cellular compartments and their benefit to living organisms
Covers general material on the inner workings of gram-negative bacteria
Offers insights into the computational methods that predict cellular localization
Presents detailed reviews of an practical hints for recombinant protein production
Table of Contents
Section I. Protein Secretion
1. Co- and Post-translational Protein Targeting to the SecYEG Translocon in Escherichia coli, Eitan Bibi
2. The Tat Protein Export Pathway, Tracy Palmer and Ben C. Berks
3. Assembly of Integral Membrane Proteins from the Periplasm into the Outer Membrane, Jörg H. Kleinschmidt
4. Structure, Function, and Transport of Lipoproteins in Escherichia coli, Hajime Tokuda, Shin-ichi Matsuyama, and Kimie Tanaka-Masuda
Section II. Protein Folding and Quality Control
5. The Cpx Envelope Stress Response, Tracy L. Raivio
6. Regulation and Function of the Envelope Stress Response Controlled by s E, Carol A. Gross, Virgil Rhodius, and Irina L. Grigorova
7. Disulfide Bond Formation in the Periplasm, Mehmet Berkmen, Dana Boyd, and Jon Beckwith
8. Periplasmic Chaperones and Peptidyl-Prolyl Isomerases, Jean-Michel Betton
9. Periplasmic Proteases and Protease Inhibitors, Nicolette Kucz, Michael Meltzer, and Michael Ehrmann
Section III. Key Physiological Processes
10. Cell Division, S. J. Ryan Arends, Kyle B. Williams, Ryan J. Kustusch, and David S. Weiss
11. Structure and Biosynthesis of the Murein (Peptidoglycan) Sacculus, Waldemar Vollmer
12. Periplasmic Events in the Assembly of Bacterial Lipopolysaccharides, Chris Whitfield, Emilisa Frirdich, and Anne N. Reid
13. Electron Transport Activities in the Periplasm, Stuart J. Ferguson
14. Periplasmic Nitrate Reduction, Jeff A. Cole
15. Biosynthesis of the Molybdenum Cofactor and its Incorporation into Molybdoenzymes, Silke Leimkühler
16. Transfer of Energy and Information across the Periplasm in Iron Transport and Regulation, Volkmar Braun and Susanne Mahren
17. Periplasmic ABC Transporters, Elie Dassa
18. Antimicrobial and Stress Resistance, Keith Poole
19. Osmoregulation in the Periplasm, Jean-Pierre Bohin and Jean-Marie Lacroix
Section IV. Practical Implications
20. Practical Implications for Periplasmic Protein Accumulation, John C. Joly and Michael W. Laird
21. Periplasmic Expression of Antibody Fragments, David P. Humphreys
Section V. Protein Composition
22. Methods for the Computational Prediction of Periplasmic Proteins, Jennifer L. Gardy and Fiona S. L. Brinkman