Kurzbeschreibung
Fundiert, verständlich, anwendungsorientiert: Im Lehrbuchstil verfasst, führt das Werk so in die Massenspektrometrie ein, dass seine Leser nach gründlicher Lektüre in der Lage sind, die hier vorgestellten Techniken - insbesondere moderne Massenspektrometer - im Labor einzusetzen.
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PREFACE
Chapter 1: MEASUREMENT, DIMENSIONS AND UNITS
1.1 INTRODUCTION
1.2 The International System of Units (SI)
Mass-to-charge ratio in mass spectrometry
1.4 ACHIEVAABLE PRECISION IN MEASUREMENT OF SI BASE QUANTITIES
1.5 MOLECULAR MASS LIMIT FOR TRACE QUANTITATION BY MASS SPECTROMETRY
Chapter 2: TOOLS OF THE TRADE I. THE CLASSICAL TOOLS
2.1 Introduction
2.2 Calibration Standards
2.3 The Analytical Balance
2.4 Measurement and Dispensing of Volume
2.5 Preparation of Solutions for Mass Spectrometer Calibration
2.6INTRODUCTION TO CALIBRATION METHODS FOR QUANTITATIVE ANALYSIS BY CHROMATOGRAPHY
Chapter 3: TOOLS OF THE TRADE II. THEORY OF CHROMATOGRAPHY
3.1 Introduction
3.2 General Principles OF Chemical Separations
3.3 SUMMARY OF IMPORTANT CONCEPTS
3.4 Plate Theory of Chromatography
3.5 Non-Equilibrium Effects in Chromatography: THE Van Deemter Equation
3.6 Gradient Elution
3.7 Capillary Electrophoresis and Capillary
Chapter 4: TOOLS OF THE TRADE III. SEPARATION PRACTICALITIES
4.1 Introduction
4.2 The Analyte and the Matrix
4.3 Extraction and Cleanup Methods
4.4 Chromatographic practicalities
4.5 SUMMARY OF KEY CONCEPTS
Chapter 5: TOOLS OF THE TRADE IV. INTERFACES AND ION SOURCES FOR CHROMATOGRAPHY-MASS SPECTROMETRY
5.1 Introduction
5.2 ION SOURCES THAT CAN REQUIRE A Discrete interface between chromatograph and source
5.3. ION SOURCES NOT REQUIRING A DISCRETE INTERFACE
5.4SOURCE-ANALYZER INTERFACES BASED ON ION MOBILITY
5.5 SUMMARY OF KEY CONCEPTS
APPENDIX 5.1 - MALDI SPOT PREPARATION TECHNIQUES
Chapter 6: TOOLS OF THE TRADE V. MASS ANALYZERS FOR QUANTITATION: SEPARATION OF IONS BY m/z VALUES
6.1 INTRODUCTION
6.2 Mass Analyzer Operation Modes and Tandem Mass Spectrometry
6.3 MOTION OF IONS IN ELECTRIC AND MAGNETIC FIELDS
6.4 MASS ANALYZERS
6.5 AcTivation and Dissociation of Ions
6.6 Vacuum systems
6.7 SUMMARY OF KEY CONCEPTS
Chapter 7: TOOLS OF THE TRADE VI: ION DETECTION
7.1. INTRODUCTION
7.2 Faraday Cup detectors
7.3 Electron multipliers
7.4 Post-detectoR electronics
Chapter 8: TOOLS OF THE TRADE VII: STATISTICS OF SAMPLING, CALIBRATION AND MEASUREMENT
INTRODUCTION
8.1 Systematic and random errors accuracy and precision
8.2 Univariate DATA: TOOLS AND TESTS FOR
8.3 DETERMINING ACCURACY AND PRECISION
8.4 Bivariate DATA: TOOLS AND TESTS FOR REGRESSION
8.5 AND CORRELATION
8.6 LIMITS OF DETECTION AND QUANTITATION
8.7 calibration and measurement: SYSTEMATIC AND RANDOM ERRORS
8.8 Statistics of sampling of heterogeneous matrices
8.9 SUMMARY OF KEY CONCEPTS
Appendix 8.1 A Brief Statistics Glossary
Appendix 8.2 Symbols used in discussion of calibration methods
Chapter 9. METHOD DEVELOPMENT AND FITNESS FOR PURPOSE
9.1 INTRODUCTION
9.2 FITNESS FOR PURPOSE AND MANAGING UNCERTAINTY
9.3 ISSUES BETWEEN ANALYST AND CLIENT: EXAMINING WHAT' AT STAKE
9.4 FORMULATING A STRATEGY
9.5 METHOD DEVELOPMENT
9.6 MATRIX EFFECTS
9.7 CONTAMINATION AND CARRYOVER
9.8 ESTABLISHING THE FINAL METHOD
Chapter 10: METHOD VALIDATION AND SAMPLE ANALYSIS
IN A CONTROLLED LABORATORY ENVIRONMENT
10.1 INTRODUCTION
10.2 METHOD VALIDATION
10.3 CONDUCT OF THE VALIDATON
10.4 EXAMPLES OF METHODS AND VALIDATIONS FIT FOR PURPOSE
10.5 VALIDATED SAMPLE ANALYIS
10.6 DOCUMENTATION
10.7 TRACEABILITY
Chapter 11: EXAMPLES FROM THE LITERATURE
INTRODUCTION
11.2 FOOD CONTAMINANTS
11.3 ANTHROPOGENIC POLLUTANTS IN WATER
11.4 GC-MS ANALYSES OF PERSISTENT ENVIRONMENTAL POLLUTANTS
11.5 BIOANALYTICAL APPLICATIONS
11.6 QUANTITATIVE PROTEOMICS
11.7 ANALYSIS OF ENDOGENOUS ANALYTES