Preface xxx
1
Light Microscopy 1
Ernst Keller
2
Confocal Microscopy, Deconvolution, and Structured Illumination Methods 43
John M. Murray
3
Multiphoton and Multispectral Laser-scanning Microscopy 83
Mary E. Dickinson
4
Preparation of Cells and Tissues for Fluorescence Microscopy 103
Andrew H. Fischer, K.A. Jacobson, J. Rose, and Rolf Zeller
5
Nonimmunological Fluorescent Labeling of Cellular Structures 123
Brad Chazotte
6
Introduction to Immunofluorescence Microscopy 143
George McNamara
7
Immunostaining of Microtubules, Microtubule-associated Proteins,
And Intermediate Filaments 155
S. Khoun and Joanna Olmsted
8
Immunofluorescence Localization of Actin 165
Anna Spudich
9
Immunofluorescence Localization of Nuclear Proteins 171
David L. Spctor
10
Immunofluorescence Methods for Saccharomyces cerevisiae 175
Pamela Silver
11
Immunofluorescence Methods for Drosophila Tissues 183
Jason Swedlow
12
Immunofluorescence Methods for Caenorhabditis elegans 193
Sarah Crittenden and Judith Kimble
13
Analyzing DNA Replication: Nonisotopic Labeling 201
Dean Jackson and Peter R. Cook
14
Analyzing RNA Synthesis: Nonisotopic Labeling 213
Dean Jackson and Peter R. Cook
15
Fluorescence In Situ Hybridization 223
Peter Lichter, Stefan Lampen, and Johanna Bridger
16
Whole-Mount Fluorescence In Situ Hybridization to
Drosophila Chromosomal DNA 261
Abby Dernburg
17
In Situ Hybridization to RNA 277
Sui Huang and Robert Singer
18
Whole-Mount In Situ Detection of RNAs in Vertebrate Embryos
and Isolated Organs 291
Anna Haramis and Rolf Zeller
19
Image Production Using Transmission Electron Microscopy 303
Mark H. Ellisman
20
Preparative Methods for Transmission Electron Microscopy 307
T. Deerinck, M. Martone, and Mark H. Ellisman
21
Immunoelectron Microscopy 343
Robert Ochs
Appendix 1
Microscopy: Lenses, Filters, and Emission/Excitation Spectra XXX
Appendix 2
Localization Markers for Subcellular Components XXX
Appendix 3
Cautions XXX
Index
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edited by David L. Spector ; Robert D. Goldman
Jahr:
2006
Verlag:
Cold Spring Harbor, NY, Cold Spring Harbor Lab. Press
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Methodenbuch
ISBN:
0879697512
Beschreibung:
IX, 382 S. : Ill., graph. Darst.
Sprache:
englisch||
Fußnote:
Literaturangaben
Mediengruppe:
Ausleihbestand