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Proximity labeling

methods and protocols
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Jahr: [2019]
Verlag: New York, NY, Humana Press, Springer Science+Business Media LLC, part of Springer Nature
Reihe: Methods in molecular biology; 2008 , Springer protocols
Mediengruppe: Dauerleihe
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Zweigstelle: Dauerleihe Standorte: ZB-130 134 Status: Entliehen Vorbestellungen: 0 Frist: 01.01.2199 Barcode: 00307270 Lagepläne: Lageplan Ausleihhinweis:

Inhalt

This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.

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Details

Suche nach diesem Verfasser
Verfasserangabe: edited by Murat Sunbul and Andres Jäschke, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany
Jahr: [2019]
Verlag: New York, NY, Humana Press, Springer Science+Business Media LLC, part of Springer Nature
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Systematik: Suche nach dieser Systematik ZB-130
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ISBN: 9781493995394
2. ISBN: 1493995367
Beschreibung: x, 231 Seiten, Illustrationen, Diagramme
Reihe: Methods in molecular biology; 2008 , Springer protocols
Schlagwörter: Zellbiologie Methoden
Beteiligte Personen: Suche nach dieser Beteiligten Person Sunbul, Murat (Herausgeber); Jäschke, Andres (Herausgeber)
Sprache: Englisch
Originaltitel: Proximity labeling
Mediengruppe: Dauerleihe