Part I. General
1. Hemagglutination Assay for Influenza Virus
Mary Lea Killian
2. Hemagglutination-Inhibition Assay for Influenza Virus Subtype Identification and the Detection and Quantitation of Serum Antibodies to Influenza Virus
Janice. C. Pedersen
3. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Virus Subtype or Neuraminidase Antibody Specificity
Janice. C. Pedersen
4. Reverse Genetics of Influenza Virus
Chang-Won Lee
5. Immunohistochemical Staining of Influenza Virus in Tissues
Mary J. Pantin-Jackwood
Part II. Avian Influenza
6. A Brief Introduction to Avian Influenza Virus
Erica Spackman
7. Wild Bird Surveillance for Avian Influenza Virus
Justin D. Brown, Rebecca Poulson, and David E. Stallknecht
8. Avian Influenza Virus Sample Types, Collection and Handling
Mary Lea Killian
9. Avian Influenza Virus RNA Extraction
Erica Spackman and Scott A. Lee
10. Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR
Erica Spackman
11. Influenza Subtype Identification with Molecular Methods
Erica Spackman
12. Avian Influenza Virus Isolation, Propagation and Titration in Embryonated Chicken Eggs
Erica Spackman and Mary Lea Killian
13. Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus
Terra A. Jenson
14. Detection of Influenza A Antibodies in Avian Serum Samples by ELISA
Len Chappell, Mary Lea Killian, and Erica Spackman
15. Conducting Influenza Virus Pathogenesis Studies in Avian Species
Carol Cardona, David A. Halvorson, Justin Brown, Jeffrey Hall, and Mary Pantin-Jackwood
16. Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry
David E. Swayne
17. Detection of Cell Mediated Immune Response to Avian Influenza Viruses
Darrell R. Kapczynski
18. Characterization of Cytokine Expression Induced by Avian Influenza Virus Infection with Real-Time RT-PCR
Darrell R. Kapczynski, Hai Jun Jiang, and Michael H. Kogut
19. Newcastle Disease Virus Detection and Differentiation from Avian Influenza
Patti J. Miller and Mia Kim Torchetti
20. A Brief Introduction to Influenza A Virus in Swine
Amy L. Vincent, Kelly M. Lager, and Tavis K. Anderson
21. Sample Types, Collection, and Transport for Influenza A Viruses of Swine
Marie Culhane and Susan Detmer
22. Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs
Jianqiang Zhang and Phillip C. Gauger
23. RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-Time RT-PCR
Jianqiang Zhang and Karen M Harmon
24. Hemagglutinin Inhibition Assay with Swine Sera
Pravina Kitikoon, Phillip C. Gauger, and Amy L. Vincent
25. Enzyme-Linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype Specific IgG and IgA Whole Virus Antibody to Influenza A Virus in Swine
Phillip C. Gauger, Crystal L. Loving, and Amy L. Vincent
26. Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine
Phillip C. Gauger and Amy L. Vincent
27. Microneutralization Assay for Swine Influenza Virus in Swine Serum
Pravina Kitikoon and Amy L. Vincent
28. Analyzing Swine Sera for Functional Antibody Titers Against Influenza A Neuraminidase Proteins Using an Enzyme-Linked Lectin Assay (ELLA)
Matthew R. Sandbulte and Maryna C. Eichelberger
29. Antibody Secreting Cell Assay for Influenza A Virus in Swine
Pravina Kitikoon, Crystal L. Loving, and Amy L. Vincent
30. In vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza
Kelly M. Lager and Amy L. Vincent
Part III. Equine Influenza
31. A Brief Introduction to Equine Influenza and Equine Influenza Viruses
Thomas M. Chambers
32. Equine Influenza Diagnosis: Sample Collection and Transport
Thomas M. Chambers and Stephanie E. Reedy
33. RNA Extraction from Equine Samples for Equine Influenza Virus
Udeni B. R. Balasuriya
34. Type A Influenza Virus Detection from Horses by Real-Time RT-PCR and Insulated Isothermal RT-PCR
Udeni B. R. Balasuriya
35. Equine Influenza Culture Methods
Thomas M. Chambers and Stephanie E. Reedy
36. Equine Influenza Serological Methods
Thomas M. Chambers and Stephanie E. Reedy