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Branch: Hauptstelle	Locations: BC-90 85	Status: available	Reservations: 0	Due date:	Barcode: 00272230	Floor plans: Floor plan	Lending note:

Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further.

The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class.

TOC:

Foreword and Introduction

Laboratory safety policies, notebook, lab report formatting & other expectations

Experiment 1: An introduction to basic math and operations in the biomolecular laboratory

Experiment 2: Buffer preparation and pH

Experiment 3: Amino acid properties and an introduction to chromatography

Experiment 4: Rapid purification, gel electrophoresis and enzyme activity assay of the firefly protein enzyme luciferase

Experiment 5: Hexokinase and G6DH catalyzed reactions of glucose measurement

Experiment 6: Polymerase chain reaction and DNA sequence analysis

Experiment 7: Investigating protein:nucleic acid interactions by electrophoretic mobility shift assay (emsa)

Experiment 8: RNA quantification and degradation kinetics

Experiment 9: Microscale preparation of liposome under mock biotechnology conditions: testing its protein interaction

Experiment 10: Preparation and characterization of a fluorescently tagged liposome-protein-DNA particle as a simple model “artificial cell”