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Table of Contents

 

Part I: Direct Microscopic Examination of Clinical Specimens

 

* Introduction

* Histological Terminology

* Tissue Reactions to Fungal Infection

* Stains

* Guide to Interpretation of Direct Microscopic Examination

* Detailed Descriptions

* Special References

 

Part II: Identification of Fungi in Culture

 

* Guide to Interpretation of Fungi in Culture

* Detailed Descriptions

 

Part III: Laboratory Technique

 

Laboratory Procedures

 

* Collection and Preparation of Specimens

* Methods for Direct Microscopic Examination of Specimens

* Primary Isolation

* Macroscopic Examination of Cultures

* Microscopic Examination of Growth

* Procedure for Identification of Yeasts

* Isolation of Yeast When Mixed with Bacteria

* Germ Tube Test for Identification of Candida albicans

* Rapid Enzyme Tests for the Presumptive Identification of Candida albicans

* Caffeic Acid Disc Test

* Olive Oil Disks for Culturing Malassezia furfur

* Conversion of Thermally Dimorphic Fungi in Culture

* Sporulation Inducement Method for Apophysomyces and Saksenaea

* In Vitro Hair Perforation Test

* Germ Tube Test for Differentiation of Some Dematiaceous Fungi

* Maitenance of Stock Fungal Cultures

* Controlling Mites

 

Staining Methods

 

* Acid-Fast Stains

* Ascospore Stain

* Calcofluor White

* Giemsa Stain

* Gomori Methenamine Silver Stain

* Gram Stain

* Lactophenol Cotton Blue

* Lactophenol Cotton Blue with PVA (Huber's Mount, Modified)

* Rehydration of Paraffin-Embedded Tissue

 

Media

 

* Ascospore Media

* Assimilation Media (for Yeasts)

* Birdseed Agar

* Brain Heart Infusion (BHI) Agar

* Candida ID Agar

* Casein Agar

* CHROMagar Candida Medium

* Cornmeal Agars

* Dermatophyte Test Medium (DTM)

* Fermentation Broths for Yeasts

* Gelatin Media

* Inhibitory Mold Agar (IMA)

* Loeffler Medium

* Lysozyme Medium

* Mycosel Agar

* Potato Dextrose Agar (PDA) and Potato Flake Agar (PFA)

* Rapid Assimilation of Trehalose (RAT) Broth

* Rapid Sporulation Medium (RSM)

* SABHI Agar

* Sabouraud Dextrose Agaar (SDA)

* Sabouraud Dextrose Agar with 15% Sodium Chloride

* Sabouraud Dextrose Broth

* Starch Hydrolysis Agar

* Trichophyton Agars

* Tyrosine or Xanthine Agar

* Urea Agar

* Water Agar

* Yeast Extract-Phosphate Agar with Ammonia