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Morpholino oligomers

methods and protocols
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Year: [2017]
Publisher: New York, NY, Humana Press
Series: Methods in molecular biology; 1565 , Springer protocols
Media group: Ausleihbestand
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Branch: Hauptstelle Locations: GE-40 321 Status: available Reservations: 0 Due date: Barcode: 00299340 Floor plans: Floor plan Lending note:

Content

This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos in utero to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier.
 

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Details

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Statement of Responsibility: Edited by Hong M. Moulton, department of Biomedical sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA, Jon D. Moulton, Gene Tools, LLC, Philomath, OR, USA
Year: [2017]
Publisher: New York, NY, Humana Press
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Classification: Search for this systematic GE-40, NA-20
Subject type: Search for this subject type Sammelwerk
ISBN: 978-1-493-96815-2
ISBN (2nd): 1-493-96815-7
Description: x, 284 Seiten : Illustrationen, Diagramme
Series: Methods in molecular biology; 1565 , Springer protocols
Tags: Genetik Methoden; Nukleinsäurebausteine; Nukleinsäuren
Participating parties: Search for this character Moulton, Hong M. [Herausgeber]; Moulton, Jon D. [Herausgeber]
Language: Englisch
Footnote: Literaturangaben
Media group: Ausleihbestand